Pseudomonas aeruginosa PAO1, PA1445 (fliO)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA1445
Name
fliO
Replicon chromosome
Genomic location 1574026 - 1574478 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1

Cross-References

RefSeq NP_250136.1
GI 15596642
Affymetrix PA1445_fliO_at
DNASU PaCD00006665
Entrez 881854
GenBank AAG04834.1
INSDC AAG04834.1
NCBI Locus Tag PA1445
protein_id(GenBank) gb|AAG04834.1|AE004574_5|gnl|PseudoCAP|PA1445
TIGR NTL03PA01446
UniParc UPI000012A9DB
UniProtKB Acc Q51467
UniProtKB ID FLIO_PSEAE
UniRef100 UniRef100_Q51467
UniRef50 UniRef50_Q51467
UniRef90 UniRef90_Q51467

Product

Feature Type CDS
Coding Frame 1
Product Name
flagellar protein FliO
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.44
Kyte-Doolittle Hydrophobicity Value 0.305
Molecular Weight (kDa) 15.8
Isoelectric Point (pI) 10.79

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Virulence Evidence

Source 1
Database VFDB : VF0273
Category Flagella
Evidence
Host Organism
Notes
Reference 14617143

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 87 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001362 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA1445
Search term: fliO

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Components of the Salmonella flagellar export apparatus and classification of export substrates.
Minamino T, Macnab RM
J Bacteriol 1999 Mar;181(5):1388-94
PubMed ID: 10049367