Pseudomonas aeruginosa PAO1, PA2494 (mexF)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 2810009 - 2813197 (+ strand)
Transposon Mutants 11 transposon mutants in PAO1
Transposon Mutants in orthologs 8 transposon mutants in orthologs


RefSeq NP_251184.1
GI 15597690
Affymetrix PA2494_mexF_at
Entrez 882884
GenBank AAG05882.1
INSDC AAG05882.1
NCBI Locus Tag PA2494
protein_id(GenBank) gb|AAG05882.1|AE004676_14|gnl|PseudoCAP|PA2494
UniParc UPI00000C5704
UniProtKB Acc Q9I0Y8
UniRef100 UniRef100_Q9I0Y8
UniRef50 UniRef50_Q8EBL9
UniRef90 UniRef90_Q9I0Y8


Feature Type CDS
Coding Frame 1
Product Name
Resistance-Nodulation-Cell Division (RND) multidrug efflux transporter MexF
Product Name Confidence: Class 1
Synonyms RND multidrug efflux transporter MexF
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -11.43
Kyte-Doolittle Hydrophobicity Value 0.284
Molecular Weight (kDa) 115.5
Isoelectric Point (pI) 5.41

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
MexF MexF is the multidrug inner membrane transporter of the MexEF-OprN complex. mexF corresponds to 2 loci in Pseudomonas aeruginosa PAO1 (gene name: mexF/mexB) and 4 loci in Pseudomonas aeruginosa LESB58 (gene name: mexD/mexB). [PMID:10515918] ARO:3000804 protein homolog model Perfect 2016.5 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 488 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000417 (2093 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2494
Search term: mexF

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC
Mol Microbiol 1997 Jan;23(2):345-54
PubMed ID: 9044268