Pseudomonas aeruginosa PAO1, PA2495 (oprN)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 2813194 - 2814612 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_251185.1
GI 15597691
Affymetrix PA2495_oprN_at
Entrez 882885
GenBank AAG05883.1
INSDC AAG05883.1
NCBI Locus Tag PA2495
protein_id(GenBank) gb|AAG05883.1|AE004676_15|gnl|PseudoCAP|PA2495
UniParc UPI00000C5705
UniProtKB Acc Q9I0Y7
UniRef100 UniRef100_W0YYH5
UniRef50 UniRef50_Q88HD3
UniRef90 UniRef90_Q02MU4


Feature Type CDS
Coding Frame 1
Product Name
Multidrug efflux outer membrane protein OprN precursor
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -6.52
Kyte-Doolittle Hydrophobicity Value -0.291
Molecular Weight (kDa) 51.2
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 3
Outer Membrane Class 1
Additional evidence for subcellular localization

AMR gene predictions from CARD database

Identified using the Resistance Gene Identifier (RGI)

Model Name Definition Accession Model Type (?) SNP Cutoff Bit Score Percent Identity CARD Version
OprN OprN is the outer membrane channel component of the MexEF-OprN multidrug efflux complex. [PMID:10515918] ARO:3000805 protein homolog model Perfect 877.5 100.0 3.2.5

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5NSW MEMBRANE PROTEIN 04/27/17 Xenon for tunnelling analysis of the efflux pump component OprN. Pseudomonas aeruginosa PAO1 2.5 X-RAY DIFFRACTION 100.0
5AZP MEMBRANE PROTEIN 10/21/15 Crystal structure of a membrane protein from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 1.69 X-RAY DIFFRACTION 100.0
5AZO MEMBRANE PROTEIN 10/21/15 Crystal structure of a membrane protein from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) 2.7 X-RAY DIFFRACTION 100.0
5IUY TRANSPORT PROTEIN 03/18/16 Structural insights of the outer-membrane channel OprN Pseudomonas aeruginosa PAO1 2.29 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 300 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG003062 (529 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA2495
Search term: oprN

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC
Mol Microbiol 1997 Jan;23(2):345-54
PubMed ID: 9044268