Pseudomonas aeruginosa PAO1, PA3133 (sawR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3133
Name
sawR
Replicon chromosome
Genomic location 3514023 - 3514565 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
CommentsawR is highly overexpressed when P. aeruginosa is exposed to Surface Acoustic Waves.
CommentSawR down-regulates the expression levels of hmgA and this causes the accumulation of the brown pigment pyomelanin. Note that SawR does not bind directly to the hmgA promoter.

Cross-References

RefSeq NP_251823.1
GI 15598329
Affymetrix PA3133_at
DNASU PaCD00007377
Entrez 880097
GenBank AAG06521.1
INSDC AAG06521.1
NCBI Locus Tag PA3133
protein_id(GenBank) gb|AAG06521.1|AE004737_7|gnl|PseudoCAP|PA3133
TIGR NTL03PA03133
UniParc UPI00000C5922
UniProtKB Acc Q9HZ91
UniProtKB ID Q9HZ91_PSEAE
UniRef100 UniRef100_Q9HZ91
UniRef50 UniRef50_Q9HZ91
UniRef90 UniRef90_Q9HZ91

Product

Feature Type CDS
Coding Frame 1
Product Name
SawR
Product Name Confidence: Class 1
Synonyms probable transcriptional regulator
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.31
Kyte-Doolittle Hydrophobicity Value 0.008
Molecular Weight (kDa) 19.2
Isoelectric Point (pI) 6.28

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2FD5 TRANSCRIPTION REGULATOR 12/13/05 The crystal structure of a transcriptional regulator from Pseudomonas aeruginosa PAO1 Pseudomonas aeruginosa 1.7 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 154 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002465 (448 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3133
Search term: sawR
Search term: SawR

Human Homologs

References

Surface acoustic waves increase the susceptibility of Pseudomonas aeruginosa biofilms to antibiotic treatment.
Kopel M, Degtyar E, Banin E
Biofouling 2011 Aug;27(7):701-10
PubMed ID: 21732715
SawR a new regulator controlling pyomelanin synthesis in Pseudomonas aeruginosa.
Ben-David Y, Zlotnik E, Zander I, Yerushalmi G, Shoshani S, Banin E
Microbiol Res 2018 Jan;206:91-98
PubMed ID: 29146265