Pseudomonas aeruginosa PAO1, PA3159 (wbpA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA3159
Name
wbpA
Replicon chromosome
Genomic location 3543764 - 3545074 (- strand)

Cross-References

RefSeq NP_251849.1
GI 15598355
Affymetrix PA3159_wbpA_at
Entrez 879921
GenBank AAG06547.1
INSDC AAG06547.1
NCBI Locus Tag PA3159
protein_id(GenBank) gb|AAG06547.1|AE004739_9|gnl|PseudoCAP|PA3159
TIGR NTL03PA03159
UniParc UPI00000DA12D
UniProtKB Acc G3XD94
UniProtKB ID UGND_PSEAE
UniRef100 UniRef100_G3XD94
UniRef50 UniRef50_G3XD94
UniRef90 UniRef90_G3XD94

Product

Feature Type CDS
Coding Frame 1
Product Name
UDP-N-acetyl-d-glucosamine 6-Dehydrogenase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Detected using LC-MS/MS (48.16 kDa, PMID:21751344)
Charge (pH 7) -10.73
Kyte-Doolittle Hydrophobicity Value -0.071
Molecular Weight (kDa) 48.2
Isoelectric Point (pI) 5.23

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database VFDB : VF0085
Category LPS O-antigen (P. aeruginosa)
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002456 (127 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3159
Search term: wbpA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B-band lipopolysaccharide gene cluster.
Burrows LL, Charter DF, Lam JS
Mol. Microbiol. 1996 Nov;22(3):481-95
PubMed ID: 8939432
Biochemical characterization of WbpA, a UDP-N-acetyl-D-glucosamine 6-dehydrogenase involved in O-antigen biosynthesis in Pseudomonas aeruginosa PAO1.
Miller WL, Wenzel CQ, Daniels C, Larocque S, Brisson JR, Lam JS
J. Biol. Chem. 2004 Sep;279(36):37551-8
PubMed ID: 15226302
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344