Pseudomonas aeruginosa PAO1, PA4710 (phuR)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4710
Name
phuR
Replicon chromosome
Genomic location 5289217 - 5291511 (+ strand)
Transposon Mutants 8 transposon mutants in PAO1
Transposon Mutants in orthologs 13 transposon mutants in orthologs

Cross-References

RefSeq NP_253398.1
GI 15599904
Affymetrix PA4710_at
Entrez 881550
GenBank AAG08096.1
INSDC AAG08096.1
NCBI Locus Tag PA4710
protein_id(GenBank) gb|AAG08096.1|AE004885_1|gnl|PseudoCAP|PA4710
TIGR NTL03PA04711
UniParc UPI00000C5DD8
UniProtKB Acc Q9HV88
UniProtKB ID Q9HV88_PSEAE
UniRef100 UniRef100_W0Z1I5
UniRef50 UniRef50_Q4K7S2
UniRef90 UniRef90_B7V1C2

Product

Feature Type CDS
Coding Frame 1
Product Name
Heme/Hemoglobin uptake outer membrane receptor PhuR precursor
Product Name Confidence: Class 1
Synonyms Haem/Haemoglobin uptake outer membrane receptor PhuR precursor
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.7, molecular weight:84.7, PMID:21674802)
Charge (pH 7) -8.64
Kyte-Doolittle Hydrophobicity Value -0.582
Molecular Weight (kDa) 84.7
Isoelectric Point (pI) 5.94

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 1
Outer Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 340 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000897 (3061 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4710
Search term: phuR

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Hemin uptake system of Yersinia enterocolitica: similarities with other TonB-dependent systems in gram-negative bacteria.
Stojiljkovic I, Hantke K
EMBO J 1992 Dec;11(12):4359-67
PubMed ID: 1425573
Characterization of the Vibrio cholerae outer membrane heme transport protein HutA: sequence of the gene, regulation of expression, and homology to the family of TonB-dependent proteins.
Henderson DP, Payne SM
J Bacteriol 1994 Jun;176(11):3269-77
PubMed ID: 8195082
Gene repression by the ferric uptake regulator in Pseudomonas aeruginosa: cycle selection of iron-regulated genes.
Ochsner UA, Vasil ML
Proc Natl Acad Sci U S A 1996 Apr 30;93(9):4409-14
PubMed ID: 8633080
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Differential contributions of the outer membrane receptors PhuR and HasR to heme acquisition in Pseudomonas aeruginosa.
Smith AD, Wilks A
J Biol Chem 2015 Mar 20;290(12):7756-66
PubMed ID: 25616666