Pseudomonas aeruginosa PAO1, PA5498 (znuA)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA5498
Name	znuA Synonym: znuA
Replicon	chromosome
Genomic location	6191186 - 6192109 (- strand)
Transposon Mutants	7 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs
Comment	Structural similarity to 2XQV (zinc transporter ZnuA from Salmonella enterica, root-mean-square deviation: 1.7 angstroms). Sequence similarity to 4BBP (98%/39,72%) (ZN). Final predicted ligand: zinc Ion (PMID: 31631799)

Cross-References

RefSeq	NP_254185.1
GI	15600691
Affymetrix	PA5498_at
Entrez	878912
GenBank	AAG08883.1
INSDC	AAG08883.1
NCBI Locus Tag	PA5498
protein_id(GenBank)	gb|AAG08883.1|AE004962_7|gnl|PseudoCAP|PA5498
TIGR	NTL03PA05499
UniParc	UPI00000C6039
UniProtKB Acc	Q9HT75
UniProtKB ID	Q9HT75_PSEAE
UniRef100	UniRef100_Q9HT75
UniRef50	UniRef50_I7C2G8
UniRef90	UniRef90_Q9HT75

Product

Feature Type	CDS
Coding Frame	1
Product Name	ZnuA Product Name Confidence: Class 1
Synonyms	probable adhesin
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602). Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7)	-5.44
Kyte-Doolittle Hydrophobicity Value	-0.086
Molecular Weight (kDa)	33.6
Isoelectric Point (pI)	6.13

Subcellular localization

Individual Mappings	Localization Confidence PMID Periplasmic Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 467 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG005037 (538 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA5498 Search term: znuA Search term: ZnuA

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen. Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM Proteomics 2009 Apr;9(7):1901-15 PubMed ID: 19333994

The transcriptional regulator Np20 is the zinc uptake regulator in Pseudomonas aeruginosa. Ellison ML, Farrow JM 3rd, Parrish W, Danell AS, Pesci EC PLoS One 2013;8(9):e75389 PubMed ID: 24086521

ZnuA and zinc homeostasis in Pseudomonas aeruginosa. Pederick VG, Eijkelkamp BA, Begg SL, Ween MP, McAllister LJ, Paton JC, McDevitt CA Sci Rep 2015 Aug 20;5:13139 PubMed ID: 26290475

Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach. Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F J Biomol Struct Dyn 2020 Sep;38(15):4508-4520 PubMed ID: 31631799