Pseudomonas aeruginosa PAO1, PA5498 (znuA)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Synonym: znuA
Replicon chromosome
Genomic location 6191186 - 6192109 (- strand)
Transposon Mutants 7 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 2XQV (zinc transporter ZnuA from Salmonella enterica, root-mean-square deviation: 1.7 angstroms). Sequence similarity to 4BBP (98%/39,72%) (ZN). Final predicted ligand: zinc Ion (PMID: 31631799)


RefSeq NP_254185.1
GI 15600691
Affymetrix PA5498_at
Entrez 878912
GenBank AAG08883.1
INSDC AAG08883.1
NCBI Locus Tag PA5498
protein_id(GenBank) gb|AAG08883.1|AE004962_7|gnl|PseudoCAP|PA5498
UniParc UPI00000C6039
UniProtKB Acc Q9HT75
UniRef100 UniRef100_Q9HT75
UniRef50 UniRef50_I7C2G8
UniRef90 UniRef90_Q9HT75


Feature Type CDS
Coding Frame 1
Product Name
Product Name Confidence: Class 1
Synonyms probable adhesin
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.44
Kyte-Doolittle Hydrophobicity Value -0.086
Molecular Weight (kDa) 33.6
Isoelectric Point (pI) 6.13

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 467 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005037 (555 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA5498
Search term: znuA
Search term: ZnuA

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
The transcriptional regulator Np20 is the zinc uptake regulator in Pseudomonas aeruginosa.
Ellison ML, Farrow JM, Farrow JM, Parrish W, Danell AS, Pesci EC
PLoS ONE 2013 ;8(9):e75389
PubMed ID: 24086521
ZnuA and zinc homeostasis in Pseudomonas aeruginosa.
Pederick VG, Eijkelkamp BA, Begg SL, Ween MP, McAllister LJ, Paton JC, McDevitt CA
Sci Rep 2015 Aug;5
PubMed ID: 26290475
Uncovering the structure and function of <i>Pseudomonas aeruginosa</i> periplasmic proteins by an <i>in silico</i> approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2019 Oct 30;:1-13
PubMed ID: 31631799