Pseudomonas aeruginosa PAO1, PA5531 (tonB1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5531
Name
tonB1
Synonym: tonB
Replicon chromosome
Genomic location 6224897 - 6225925 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_254218.1
GI 15600724
Affymetrix PA5531_tonB_at
Entrez 878138
GenBank AAG08916.1
INSDC AAG08916.1
NCBI Locus Tag PA5531
protein_id(GenBank) gb|AAG08916.1|AE004965_8|gnl|PseudoCAP|PA5531
TIGR NTL03PA05532
UniParc UPI0000137125
UniProtKB Acc Q51368
UniProtKB ID TONB_PSEAE
UniRef100 UniRef100_Q51368
UniRef50 UniRef50_Q51368
UniRef90 UniRef90_Q51368

Product

Feature Type CDS
Coding Frame 1
Product Name
TonB1
Product Name Confidence: Class 1
Synonyms TonB
Evidence for Translation
Charge (pH 7) 3.92
Kyte-Doolittle Hydrophobicity Value -0.708
Molecular Weight (kDa) 36.9
Isoelectric Point (pI) 9.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Unknown Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6FIP TRANSPORT PROTEIN 01/19/18 Solution NMR structure of Pseudomonas aeruginosa TonB CTD Pseudomonas aeruginosa NOT SOLUTION NMR 100.0
6I97 MEMBRANE PROTEIN 11/22/18 Structure of the ferrioxamine B transporter FoxA from Pseudomonas aeruginosa in complex with ferrioxamine B and a C-terminal TonB fragment Pseudomonas aeruginosa 3.35 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database PseudoCAP
Category
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Mus musculus
Notes
Infection model Innoculation into immunosupressed mice.
Reference 10899848

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 99 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005064 (357 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5531
Search term: tonB1
Search term: TonB1

Human Homologs

References

The Pseudomonas aeruginosa tonB gene encodes a novel TonB protein.
Poole K, Zhao Q, Neshat S, Heinrichs DE, Dean CR
Microbiology (Reading) 1996 Jun;142 ( Pt 6):1449-1458
PubMed ID: 8704984
Role of TonB1 in pyoverdine-mediated signaling in Pseudomonas aeruginosa.
Shirley M, Lamont IL
J Bacteriol 2009 Sep;191(18):5634-40
PubMed ID: 19592589