Pseudomonas aeruginosa PAO1, PA0002 (dnaN)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 2056 - 3159 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_064722.1
GI 15595200
Affymetrix PA0002_dnaN_at
DNASU PaCD00006004
Entrez 879244
GenBank AAG03392.1
INSDC AAG03392.1
NCBI Locus Tag PA0002
protein_id(GenBank) gb|AAG03392.1|AE004440_2|gnl|PseudoCAP|PA0002
UniParc UPI0000129706
UniProtKB Acc Q9I7C4
UniRef100 UniRef100_Q9I7C4
UniRef50 UniRef50_Q9I7C4
UniRef90 UniRef90_Q9I7C4


Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, beta chain
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.60
Kyte-Doolittle Hydrophobicity Value -0.147
Molecular Weight (kDa) 40.7
Isoelectric Point (pI) 4.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6PTH TRANSFERASE/ANTIBIOTIC 07/15/19 Crystal structure of a DnaN sliding clamp (DNA polymerase III subunit beta) from Pseudomonas aeruginosa bound to griselimycin Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.05 X-RAY DIFFRACTION 100.0
4TR8 DNA BINDING PROTEIN 06/15/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa Pseudomonas aeruginosa HB15 1.8 X-RAY DIFFRACTION 100.0
6AMS TRANSFERASE 08/11/17 Crystal structure of the DNA polymerase III subunit beta from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.39 X-RAY DIFFRACTION 100.0
4TSZ TRANSFERASE 06/19/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa with ligand Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000002 (555 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0002
Search term: dnaN

Human Homologs


The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.
Burgers PM, Kornberg A, Sakakibara Y
Proc Natl Acad Sci U S A 1981 Sep;78(9):5391-5
PubMed ID: 6458041
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y
Gene 1984 May;28(2):159-70
PubMed ID: 6234204
Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
Kong XP, Onrust R, O'Donnell M, Kuriyan J
Cell 1992 May 1;69(3):425-37
PubMed ID: 1349852