Pseudomonas aeruginosa PAO1, PA0002 (dnaN)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0002
Name
dnaN
Replicon chromosome
Genomic location 2056 - 3159 (+ strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_064722.1
GI 15595200
Affymetrix PA0002_dnaN_at
DNASU PaCD00006004
Entrez 879244
GenBank AAG03392.1
INSDC AAG03392.1
NCBI Locus Tag PA0002
protein_id(GenBank) gb|AAG03392.1|AE004440_2|gnl|PseudoCAP|PA0002
TIGR NTL03PA00003
UniParc UPI0000129706
UniProtKB Acc Q9I7C4
UniProtKB ID DPO3B_PSEAE
UniRef100 UniRef100_Q9I7C4
UniRef50 UniRef50_Q9I7C4
UniRef90 UniRef90_Q9I7C4

Product

Feature Type CDS
Coding Frame 1
Product Name
DNA polymerase III, beta chain
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -10.60
Kyte-Doolittle Hydrophobicity Value -0.147
Molecular Weight (kDa) 40.7
Isoelectric Point (pI) 4.89

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6PTH TRANSFERASE/ANTIBIOTIC 07/15/19 Crystal structure of a DnaN sliding clamp (DNA polymerase III subunit beta) from Pseudomonas aeruginosa bound to griselimycin Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1); SYNTHETIC CONSTRUCT 3.05 X-RAY DIFFRACTION 100.0
4TR8 DNA BINDING PROTEIN 06/15/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa Pseudomonas aeruginosa HB15 1.8 X-RAY DIFFRACTION 100.0
6AMS TRANSFERASE 08/11/17 Crystal structure of the DNA polymerase III subunit beta from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.39 X-RAY DIFFRACTION 100.0
4TSZ TRANSFERASE 06/19/14 Crystal structure of DNA polymerase sliding clamp from Pseudomonas aeruginosa with ligand Pseudomonas aeruginosa; SYNTHETIC CONSTRUCT 2 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 588 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000002 (555 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0002
Search term: dnaN

Human Homologs

References

The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.
Burgers PM, Kornberg A, Sakakibara Y
Proc Natl Acad Sci U S A 1981 Sep;78(9):5391-5
PubMed ID: 6458041
Structural analysis of the dnaA and dnaN genes of Escherichia coli.
Ohmori H, Kimura M, Nagata T, Sakakibara Y
Gene 1984 May;28(2):159-70
PubMed ID: 6234204
Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
Kong XP, Onrust R, O'Donnell M, Kuriyan J
Cell 1992 May 1;69(3):425-37
PubMed ID: 1349852