Pseudomonas aeruginosa PAO1, PA0011 (htrB1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0011
Name
htrB1
Synonym: 2-OH-lauroyltransferase
Replicon chromosome
Genomic location 14235 - 15122 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248701.1
GI 15595209
Affymetrix PA0011_at
Entrez 877921
GenBank AAG03401.1
INSDC AAG03401.1
NCBI Locus Tag PA0011
protein_id(GenBank) gb|AAG03401.1|AE004441_2|gnl|PseudoCAP|PA0011
TIGR NTL03PA00012
UniParc UPI00000C4EE6
UniProtKB Acc Q9I7B5
UniProtKB ID Q9I7B5_PSEAE
UniRef100 UniRef100_W0YKN8
UniRef50 UniRef50_C3KE43
UniRef90 UniRef90_W0YKN8

Product

Feature Type CDS
Coding Frame 1
Product Name
acyltransferase HtrB1
Product Name Confidence: Class 1
Synonyms HtrB1
2-OH-lauroyltransferase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 11.60
Kyte-Doolittle Hydrophobicity Value -0.094
Molecular Weight (kDa) 33.0
Isoelectric Point (pI) 9.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 281 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000011 (555 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0011
Search term: htrB1

Human Homologs

References

Biosynthesis of lipid A in Escherichia coli. Acyl carrier protein-dependent incorporation of laurate and myristate.
Brozek KA, Raetz CR
J Biol Chem 1990 Sep 15;265(26):15410-7
PubMed ID: 2203778
Structural characterization of the lipid A component of Pseudomonas aeruginosa wild-type and rough mutant lipopolysaccharides.
Kulshin VA, Zähringer U, Lindner B, Jäger KE, Dmitriev BA, Rietschel ET
Eur J Biochem 1991 Jun 15;198(3):697-704
PubMed ID: 1904818
Endotoxin biosynthesis in Pseudomonas aeruginosa: enzymatic incorporation of laurate before 3-deoxy-D-manno-octulosonate.
Mohan S, Raetz CR
J. Bacteriol. 1994 Nov;176(22):6944-51
PubMed ID: 7961456
Characterization of a novel gene related to antibiotic susceptibility in Pseudomonas aeruginosa.
Shen L, Ma Y, Liang H
J. Antibiot. 2012 Feb;65(2):59-65
PubMed ID: 22146126
Function of the htrB high temperature requirement gene of Escherichia coli in the acylation of lipid A: HtrB catalyzed incorporation of laurate.
Clementz T, Bednarski JJ, Raetz CR
J. Biol. Chem. 1996 May;271(20):12095-102
PubMed ID: 8662613