Pseudomonas aeruginosa PAO1, PA0018 (fmt)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0018
Name
fmt
Replicon chromosome
Genomic location 20068 - 21012 (- strand)

Cross-References

RefSeq NP_248708.1
GI 15595216
Affymetrix PA0018_fmt_at
Entrez 879258
GenBank AAG03408.1
NCBI Locus Tag PA0018
protein_id(GenBank) gb|AAG03408.1|AE004441_9|gnl|PseudoCAP|PA0018
TIGR NTL03PA00019
UniProtKB Acc O85732
UniProtKB ID FMT_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
methionyl-tRNA formyltransferase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -0.17
Kyte-Doolittle Hydrophobicity Value 0.068
Molecular Weight (kDa) 33.0
Isoelectric Point (pI) 6.96

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5UAI TRANSFERASE 12/19/16 Crystal structure of Methionyl-tRNA formyltransferase from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.75 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 600 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000018 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0018
Search term: fmt

Human Homologs

References

Disruption of the gene for Met-tRNA(fMet) formyltransferase severely impairs growth of Escherichia coli.
Guillon JM, Mechulam Y, Schmitter JM, Blanquet S, Fayat G
J Bacteriol 1992 Jul;174(13):4294-301
PubMed ID: 1624424
The Escherichia coli fmt gene, encoding methionyl-tRNA(fMet) formyltransferase, escapes metabolic control.
Meinnel T, Guillon JM, Mechulam Y, Blanquet S
J. Bacteriol. 1993 Feb;175(4):993-1000
PubMed ID: 8432722
Structure of crystalline Escherichia coli methionyl-tRNA(f)Met formyltransferase: comparison with glycinamide ribonucleotide formyltransferase.
Schmitt E, Blanquet S, Mechulam Y
EMBO J. 1996 Sep;15(17):4749-58
PubMed ID: 8887566