Pseudomonas aeruginosa PAO1, PA0019 (def)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0019
Name
def
Replicon chromosome
Genomic location 21067 - 21573 (- strand)

Cross-References

RefSeq NP_248709.1
GI 15595217
Affymetrix PA0019_def_at
DNASU PaCD00006011
Entrez 879306
GenBank AAG03409.1
INSDC AAG03409.1
NCBI Locus Tag PA0019
protein_id(GenBank) gb|AAG03409.1|AE004441_10|gnl|PseudoCAP|PA0019
TIGR NTL03PA00020
UniParc UPI000012915C
UniProtKB Acc Q9I7A8
UniProtKB ID DEF_PSEAE
UniRef100 UniRef100_Q9I7A8
UniRef50 UniRef50_Q9I7A8
UniRef90 UniRef90_Q9I7A8

Product

Feature Type CDS
Coding Frame 1
Product Name
polypeptide deformylase
Product Name Confidence: Class 2
Synonyms formylmethionine deformylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.13
Kyte-Doolittle Hydrophobicity Value -0.441
Molecular Weight (kDa) 19.4
Isoelectric Point (pI) 4.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1N5N HYDROLASE 11/06/02 Crystal Structure of Peptide Deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 99.4
1LRY HYDROLASE 05/16/02 Crystal Structure of P. aeruginosa Peptide Deformylase Complexed with Antibiotic Actinonin Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0
1S17 HYDROLASE 01/05/04 Identification of Novel Potent Bicyclic Peptide Deformylase Inhibitors Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 99.4
1IX1 HYDROLASE 06/07/02 Crystal Structure of P.aeruginosa Peptide deformylase Complexed with Antibiotic Actinonin Pseudomonas aeruginosa 1.85 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
2-[(Formyl-Hydroxy-Amino)-Methyl]-Heptanoic Acid [1-(2-Hydroxymethyl-Pyrrolidine-1-Carbonyl)-2-Methyl-Propyl]-Amide DB04310 DrugBank 5.1.4 Q9I7A8 Peptide deformylase 100.0 168 1.5e-91
2-(3,4-Dihydro-3-Oxo-2h-Benzo[B][1,4]Thiazin-2-Yl)-N-Hydroxyacetamide DB02036 DrugBank 5.1.4 Q9I7A8 Peptide deformylase 100.0 168 1.5e-91
N-(2-Acetamido)Iminodiacetic Acid DB02810 DrugBank 5.1.4 Q9I7A8 Peptide deformylase 100.0 168 1.5e-91

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000019 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0019
Search term: def

Human Homologs

References

A new subclass of the zinc metalloproteases superfamily revealed by the solution structure of peptide deformylase.
Meinnel T, Blanquet S, Dardel F
J. Mol. Biol. 1996 Sep;262(3):375-86
PubMed ID: 8845003