Pseudomonas aeruginosa PAO1, PA0019 (def)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 21067 - 21573 (- strand)


RefSeq NP_248709.1
GI 15595217
Affymetrix PA0019_def_at
DNASU PaCD00006011
Entrez 879306
GenBank AAG03409.1
INSDC AAG03409.1
NCBI Locus Tag PA0019
protein_id(GenBank) gb|AAG03409.1|AE004441_10|gnl|PseudoCAP|PA0019
UniParc UPI000012915C
UniProtKB Acc Q9I7A8
UniRef100 UniRef100_Q9I7A8
UniRef50 UniRef50_Q9I7A8
UniRef90 UniRef90_Q9I7A8


Feature Type CDS
Coding Frame 1
Product Name
polypeptide deformylase
Product Name Confidence: Class 2
Synonyms formylmethionine deformylase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -8.13
Kyte-Doolittle Hydrophobicity Value -0.441
Molecular Weight (kDa) 19.4
Isoelectric Point (pI) 4.73

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
1S17 HYDROLASE 01/05/04 Identification of Novel Potent Bicyclic Peptide Deformylase Inhibitors Pseudomonas aeruginosa 1.95 X-RAY DIFFRACTION 99.4
1N5N HYDROLASE 11/06/02 Crystal Structure of Peptide Deformylase from Pseudomonas aeruginosa Pseudomonas aeruginosa 1.8 X-RAY DIFFRACTION 99.4
1IX1 HYDROLASE 06/07/02 Crystal Structure of P.aeruginosa Peptide deformylase Complexed with Antibiotic Actinonin Pseudomonas aeruginosa 1.85 X-RAY DIFFRACTION 100.0
1LRY HYDROLASE 05/16/02 Crystal Structure of P. aeruginosa Peptide Deformylase Complexed with Antibiotic Actinonin Pseudomonas aeruginosa 2.6 X-RAY DIFFRACTION 100.0

Drugs Targeting this Protein

Identified by Diamond using e-value cutoff of 0.0001 and returning alignments that span 100% of the query sequence and that have more than 95% identity.

Drug Name Source Accession Source DB Version Target Accession Target Description Percent Identity Alignment Length E-Value
N-(2-Acetamido)Iminodiacetic Acid DB02810 DrugBank 5.1.4 Q9I7A8 Peptide deformylase 100.0 168 1.5e-91
2-(3,4-Dihydro-3-Oxo-2h-Benzo[B][1,4]Thiazin-2-Yl)-N-Hydroxyacetamide DB02036 DrugBank 5.1.4 Q9I7A8 Peptide deformylase 100.0 168 1.5e-91
2-[(Formyl-Hydroxy-Amino)-Methyl]-Heptanoic Acid [1-(2-Hydroxymethyl-Pyrrolidine-1-Carbonyl)-2-Methyl-Propyl]-Amide DB04310 DrugBank 5.1.4 Q9I7A8 Peptide deformylase 100.0 168 1.5e-91

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 586 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000019 (537 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0019
Search term: def

Human Homologs


A new subclass of the zinc metalloproteases superfamily revealed by the solution structure of peptide deformylase.
Meinnel T, Blanquet S, Dardel F
J Mol Biol 1996 Sep 27;262(3):375-86
PubMed ID: 8845003