Pseudomonas aeruginosa PAO1, PA0024 (hemF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0024
Name
hemF
Replicon chromosome
Genomic location 25736 - 26653 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_248714.1
GI 15595222
Affymetrix PA0024_hemF_at
Entrez 880901
GenBank AAG03414.1
INSDC AAG03414.1
NCBI Locus Tag PA0024
protein_id(GenBank) gb|AAG03414.1|AE004442_1|gnl|PseudoCAP|PA0024
TIGR NTL03PA00025
UniParc UPI000012C4B5
UniProtKB Acc P43898
UniProtKB ID HEM6_PSEAE
UniRef100 UniRef100_Q02V57
UniRef50 UniRef50_P84155
UniRef90 UniRef90_A6UX86

Product

Feature Type CDS
Coding Frame 1
Product Name
coproporphyrinogen III oxidase, aerobic
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -7.53
Kyte-Doolittle Hydrophobicity Value -0.507
Molecular Weight (kDa) 34.8
Isoelectric Point (pI) 5.78

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 279 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000024 (554 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0024
Search term: hemF

Human Homologs

Ensembl 107, assembly GRCh38.p13
coproporphyrinogen oxidase [Source:HGNC Symbol;Acc:HGNC:2321]
E-value: 4.3e-75
Percent Identity: 44.4

References

An oxygen-dependent coproporphyrinogen oxidase encoded by the hemF gene of Salmonella typhimurium.
Xu K, Elliott T
J. Bacteriol. 1993 Aug;175(16):4990-9
PubMed ID: 8349542
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994