Pseudomonas aeruginosa UCBPP-PA14, PA14_00560 (exoT)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14 (Lee et al., 2006)
Locus Tag
Replicon chromosome
Genomic location 57148 - 58476 (+ strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 2 transposon mutants in orthologs


RefSeq YP_788201.1
GI 116053766
Entrez 4381738
INSDC ABJ15002.1
NCBI Locus Tag PA14_00560
UniParc UPI0000E44880
UniProtKB Acc Q02V35
UniRef100 UniRef100_Q02V35
UniRef50 UniRef50_Q93Q17
UniRef90 UniRef90_Q9I788


Feature Type CDS
Coding Frame 1
Product Name
exoenzyme T
Evidence for Translation
Charge (pH 7) -9.33
Kyte-Doolittle Hydrophobicity Value -0.313
Molecular Weight (kDa) 46.8
Isoelectric Point (pI) 5.49

Subcellular localization

Individual Mappings
Localization Confidence PMID
Extracellular Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6GNN TOXIN 05/31/18 Exoenzyme T from Pseudomonas aeruginosa in complex with human 14-3-3 protein beta, tetrameric crystal form bound to STO1101 Homo sapiens; Pseudomonas aeruginosa PAO1 3.79 X-RAY DIFFRACTION 99.5
4JMF TOXIN/CHAPERONE 03/14/13 Crystal structure of ExoT (residues 28 -77)- SpcS complex from Pseudomonas aeruginosa at 2.1 angstrom Pseudomonas aeruginosa 2.099 X-RAY DIFFRACTION 100.0
6JNP TOXIN 03/17/19 Structure of ExoT-SpcS Complex from Pseudomonas aeruginosa in 2.2 Angstrom Pseudomonas aeruginosa 2.261 X-RAY DIFFRACTION 98.2

Virulence Evidence

Source 1
Database PseudoCAP
Category T3SS translocated effectors
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Galleria mellonella
Infection model Galleria mellonella killing assay
Reference 12704110
Source 2
Database Victors : 4411
Category T3SS translocated effectors
Evidence ECO:0000314
direct assay evidence used in manual assertion
Host Organism Cricetulus griseus
Notes exoT interferes with Rho signal transduction pathways, which regulate actin organization, exocytosis, cell cycle progression, and phagocytosis
Reference 10992524

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 8 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000044 (294 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA14_00560
Search term: exoT
Search term: exoenzyme T

Human Homologs


No references are associated with this feature.