Pseudomonas aeruginosa UCBPP-PA14, PA14_72450 (dsbA1 )

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa UCBPP-PA14
GCF_000014625.1|latest
Locus Tag
PA14_72450
Name
dsbA1
Synonym: PadsbA1
Synonym: dsbA
Replicon chromosome
Genomic location 6453853 - 6454488 (- strand)
Transposon Mutants 2 transposon mutants in UCBPP-PA14
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq YP_793966.1
GI 116053639
Entrez 4385158
INSDC ABJ14875.1
NCBI Locus Tag PA14_72450
UniParc UPI00001298C0
UniProtKB Acc Q02DM0
UniProtKB ID DSBA_PSEAB
UniRef100 UniRef100_Q02DM0
UniRef50 UniRef50_O52376
UniRef90 UniRef90_Q02DM0

Product

Feature Type CDS
Coding Frame 1
Product Name
DsbA1
Product Name Confidence: Class 1
Synonyms thiol:disulfide interchange protein DsbA
Evidence for Translation
Charge (pH 7) -2.38
Kyte-Doolittle Hydrophobicity Value -0.064
Molecular Weight (kDa) 23.4
Isoelectric Point (pI) 6.40

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5TLQ OXIDOREDUCTASE 10/11/16 Model structure of the oxidized PaDsbA1 and 3-[(2-methylbenzyl)sulfanyl]-4H-1,2,4-triazol-4-amine complex PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0
5DCH OXIDOREDUCTASE 08/24/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I in complex with MIPS-0000851 (3-[(2-METHYLBENZYL)SULFANYL]-4H-1,2,4-TRIAZOL-4-AMINE) PSEUDOMONAS AERUGINOSA 1.447 X-RAY DIFFRACTION 98.4
4ZL8 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal II PSEUDOMONAS AERUGINOSA 1.395 X-RAY DIFFRACTION 98.4
3H93 TRANSCRIPTION REGULATOR 04/29/09 Crystal Structure of Pseudomonas aeruginosa DsbA PSEUDOMONAS AERUGINOSA PAO1 1.501 X-RAY DIFFRACTION 99.0
4ZL9 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal III PSEUDOMONAS AERUGINOSA 1.7 X-RAY DIFFRACTION 98.4
2MBT OXIDOREDUCTASE 08/03/13 NMR study of PaDsbA PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0
4ZL7 OXIDOREDUCTASE 05/01/15 Crystal structure of Pseudomonas aeruginosa DsbA E82I: Crystal I PSEUDOMONAS AERUGINOSA 1.922 X-RAY DIFFRACTION 98.4

Virulence Evidence

Source 1
Database Victors : 4425
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Mus musculus
Notes
Reference 18591226
Source 2
Database Victors : 4425
Category
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Drosophila melanogaster
Notes
Reference 18591226

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 149 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005029 (551 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA14_72450
Search term: dsbA1
Search term: DsbA1

Human Homologs

References

Dissecting the machinery that introduces disulfide bonds in Pseudomonas aeruginosa.
Arts IS, Ball G, Leverrier P, Garvis S, Nicolaes V, Vertommen D, Ize B, Tamu Dufe V, Messens J, Voulhoux R, Collet JF
MBio 2013 Dec;4(6):e00912-13
PubMed ID: 24327342