Pseudomonas aeruginosa PAO1, PA2492 (mexT)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA2492
Name
mexT
Replicon chromosome
Genomic location 2807469 - 2808512 (+ strand)
Transposon Mutants 5 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs
Comment PAO1 strains containing the 8_bp insertion are unable to express MexEF-OprN due to a non-functional MexT(PMID: 11040437)
CommentContains an 8_bp insertion at 2807703-2807710 which is the result of a duplication of the preceding 8_bp GGCCAGCC sequence at 2807695-2807702. When translated from this start codon, premature termination occurs at 2807857-2807859 and a MexT polypeptide of 89 amino acids is encoded (PMID:31859619).

Cross-References

RefSeq
GI
Affymetrix PA2492_mexT_at
Entrez 880417
GenBank AAG05880.1
NCBI Locus Tag PA2492
protein_id(GenBank) gb|AAG05880.1|AE004676_12|gnl|PseudoCAP|PA2492
TIGR NTL03PA02492
UniProtKB Acc Q9I0Z0
UniProtKB ID Q9I0Z0_PSEAE

Product

Feature Type CDS
Coding Frame 1
Product Name
transcriptional regulator MexT
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)
Kyte-Doolittle Hydrophobicity Value
Molecular Weight (kDa)
Isoelectric Point (pI)

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 185 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001179 (1988 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2492
Search term: mexT

Human Homologs

References

Characterization of MexE-MexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa.
Köhler T, Michéa-Hamzehpour M, Henze U, Gotoh N, Curty LK, Pechère JC
Mol. Microbiol. 1997 Jan;23(2):345-54
PubMed ID: 9044268
MexT regulates the type III secretion system through MexS and PtrC in Pseudomonas aeruginosa.
Jin Y, Yang H, Qiao M, Jin S
J. Bacteriol. 2011 Jan;193(2):399-410
PubMed ID: 21075931
<i>Pseudomonas aeruginosa mexT</i> is an indicator of PAO1 strain integrity.
LoVullo ED, Schweizer HP
J Med Microbiol 2020 Jan;69(1):139-145
PubMed ID: 31859619
Variation of the mexT gene, a regulator of the MexEF-oprN efflux pump expression in wild-type strains of Pseudomonas aeruginosa.
Maseda H, Saito K, Nakajima A, Nakae T
FEMS Microbiol Lett 2000 Nov 1;192(1):107-12
PubMed ID: 11040437