Pseudomonas aeruginosa PAO1, PA3246 (rluA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3246
Name
rluA
Synonym: yabO
Replicon chromosome
Genomic location 3632788 - 3633423 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251936.1
GI 15598442
Affymetrix PA3246_rluA_at
Entrez 882409
GenBank AAG06634.1
INSDC AAG06634.1
NCBI Locus Tag PA3246
protein_id(GenBank) gb|AAG06634.1|AE004747_7|gnl|PseudoCAP|PA3246
TIGR NTL03PA03246
UniParc UPI00000C5970
UniProtKB Acc Q9HYZ4
UniProtKB ID Q9HYZ4_PSEAE
UniRef100 UniRef100_W0YV36
UniRef50 UniRef50_A4SR76
UniRef90 UniRef90_S6ASJ3

Product

Feature Type CDS
Coding Frame 1
Product Name
pseudouridine synthase RluA
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 0.24
Kyte-Doolittle Hydrophobicity Value -0.470
Molecular Weight (kDa) 24.3
Isoelectric Point (pI) 7.04

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 591 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002362 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3246
Search term: rluA

Human Homologs

Ensembl 110, assembly GRCh38.p14
RNA pseudouridine synthase D4 [Source:HGNC Symbol;Acc:HGNC:25898]
E-value: 2.1e-15
Percent Identity: 33.0

References

A dual-specificity pseudouridine synthase: an Escherichia coli synthase purified and cloned on the basis of its specificity for psi 746 in 23S RNA is also specific for psi 32 in tRNA(phe).
Wrzesinski J, Nurse K, Bakin A, Lane BG, Ofengand J
RNA 1995 Jun;1(4):437-48
PubMed ID: 7493321