Additional resources for Pseudomonas aeruginosa sequence analysis, gene annotation and functional pathway analysis are listed below. We encourage researchers to contact us with suggestions for additional items that should be listed.
Pseudomonas genome projects at NCBI BioProject
View a list of genome and transcriptome projects in this database.
View a list at the Genomes Online website
View listing of complete and incomplete microbial genome projects at NCBI.
Transposon Mutant Library Databases
Pseudomonas aeruginosa PAO1 mini-Tn5 lux transposon mutant library (developed by the Hancock Laboratory, UBC)
Pseudomonas aeruginosa PAO1 phoA/lacZ transposon library generated by the University of Washington Genome Centre
Pseudomonoas aeruginosa PA14 non-redundant transposon insertion library developed by Ausubel lab at Harvard Medical School.
HoPaCI-DB: host-Pseudomonas and Coxiella interaction database
The host-Pseudomonas and Coxiella interaction database (HoPaCI-DB) is a publicly available manually curated integrative database of host–pathogen interaction data from Pseudomonas aeruginosa and Coxiella burnetii.
Pseudomonas aeruginosa PAO1 Protein Interactome
Based on the following publication:
Zhang M, Su S, Bhatnagar RK, Hassett DJ, Lu LJ. Prediction and analysis of the protein interactome in Pseudomonas aeruginosa to enable network-based drug target selection. PLoS One. 2012;7(7):e41202. Epub 2012 Jul 24. PubMed PMID: 22848443; PubMed Central PMCID: PMC3404098.
A website available at http://research.cchmc.org/PPIdatabase/index.html hosts the data and details of the analysis. It provides an interface to enter a protein of interest and view a list of predicted interactions.
oprD, oprI, oprL allele data
Jean-Paul Pirnay, Laboratory for Molecular and Cellular Technology, Queen Astrid Military Hospital, Brussels Belgium.
Sequenced from a collection of 328 diverse P. aeruginosa strains isolated by 69 institutes in 30 countries (5 continents), from diverse sources (river , pond and sea water, plants, animals and human CF, burns etc.) between 1882-2007.
For Pseudomonas aeruginosa (PAO1), P.syringae (DC3000,
B728A, 1448A), P. fluorescens (Pf-5, PfO-1) and P.putida (KT2440)
IslandPath is a graphical tool that aids with identification of genomics islands, including pathogenicity islands, of potentially horizontally transferred genes. Brinkman lab, Simon Fraser University, Burnaby, BC.
IslandViewer is tool for visualization of genomic islands that have been predicted using three different computational methods (IslandPick, SIGI-HMM and IslandPath-DIMOB).
Hosted by the Brinkman Lab, Simon Fraser University, Canada
WebACT provides a database of sequence comparisons between all publicly available prokaryotic genome sequences, allowing the on-line visualisation of comparisons between up to five genomic sequences, using the Artemis Comparison Tool (ACT) developed by the Sanger Institute. Abbott JC, Aanensen DM, Bentley SD. 2007 (Pubmed)
Hosted by Imperial College, London. (website)
Ribonucleotide reductase database (RNRdb)
Department of Molecular Biology, Stockholm University, Stockholm, Sweden. (website)
Genome-scale collection of Pseudomonas ORFs in recombinational cloning vectors - Harvard Institute of Proteomics at Harvard Medical School.
PseuRECA (Pseudomonas REannotation and Context Analysis) - Klinische Forschergruppe, Hannover, Germany; The European Bioinformatics Institute (EMBL), Cambridge , UK; The Institute for Genomic Research, Rockville, MD , USA
NCBI's Pseudomonas aeruginosa genome information - National Center for Biotechnology Information, Bethesda, Maryland, USA
Genome Atlas - Oak Ridge National Laboratory, Oak Ridge, TN, USA
Microbial Genome Database for Comparative Analysis - University of Tokyo, Tokyo, Japan
NCBI BLAST against Pseudomonas aeruginosa, Pseudomonas putida, and others - National Center for Biotechnology Information, Bethesda, Maryland, USA
Search PubMed for recent Pseudomonas aeruginosa-related publications - National Center for Biotechnology Information, Bethesda, Maryland, USA
KEGG: Kyoto Encyclopedia of Genes and Genomes Minoru Kanehisa, Institute for Chemical Research, Kyoto University, Kyoto, Japan
ERGO Integrated Genomics, Inc., Chicago, Illinois, USA
Codon Usage Database - Pseudomonas aeruginosa - Yasukazu Nakamura, First Laboratory for Plant Gene Research, Kazusa DNA Research Institute, Chiba, Japan
Pseudomonas Genomes at PEDANT - Dmitrij Frishman, Munich Information Center for Protein Sequences, Am Klopferspitz, Germany
PRODORIC: Prokaryotic Database of Gene Regulation and Regulatory Networks (includes P. aeruginosa regulons) - Technical University of Braunschweig, Braunschweig, Germany
Alginate Genes Kalai Mathee, Department of Biological Sciences, Florida International University, Miami, Florida, USA; Department of Clinical Microbiology, Copenhagen University Hospital, Copenhagen, Denmark
Genetic Comparisons of Membrane Transport Systems Ian Paulsen, The Institute for Genomic Research, Rockville, Maryland, USA
Outer Membrane Proteins Robert E.W. Hancock, Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada
ASM Conference on Pseudomonas - Seattle, Washington, USA (August 26-30, 2007).
Control strains for the microbiological testing of water samples for Pseudomonas aeruginosa - Public Health England Culture Collections
Pseudomonas aeruginosa (and other species) Antimicrobial Susceptibility Controls - Public Health England Culture Collections
PHE's National Collection of Type Cultures (NCTC) using Pacific Biosciences' Single Molecule, Real-Time (SMRT) sequencing technology - Wellcome Trust Sanger Institute NCTC Downloads Page
Other Related Databases
For those interested in pathogens of relevance to Cystic Fibrosis, note that a sister database for Burkholderia has been recently created. You can access it at burkholderia.com.
Other Pseudomonas species genome annotations
Pseudomonas aeruginosa PA14 genome sequencing project home page - Massachusetts General Hospital, Harvard Medical School
Pseudomonas aeruginosa PA7 genome page - J. Craig Venter Institute (JCVI), Microbial Sequencing Center (MSC)
Pseudomonas fluorescens Pf-5 genome page - Institute for Genomic Research (TIGR)
Pseudomonas fluorescens PfO-1 genome page - DOE Joint Genome Institution (JGI), The Regents of the University of California
Pseudomonas mendocina ymp genome page - DOE Joint Genome Institution (JGI), The Regents of the University of California
Pseudomonas putida F1 genome page - DOE Joint Genome Institution (JGI), The Regents of the University of California
Pseudomonas putida KT2440 genome page - Institute for Genomic Research (TIGR)
Pseudomonas stutzeri A1501 - While this link is to the annotation at NCBI, the sequencing centers are The Biotechnology Research Institute and The Chinese Academy of Agricultural Sciences. Annotation has not been added to the web site yet.
Pseudomonas syringae DC3000 annotation project - Browse the current annotation for Pseudomonas syringae DC3000 using the Artemis genome viewer. Includes a portal for submitting annotation updates and viewing its annotation at NCBI.
Pseudomonas syringae B728a annotation project - Browse the current annotation for Pseudomonas syringae B728a using the Artemis genome viewer. Includes a portal for submitting annotation updates and viewing its annotation at NCBI.
Pseudomonas syringae phaseolicola 1448A annotation project - Browse the current annotation forPseudomonas syringae phaseolicola using the Artemis genome viewer. Includes a portal for submitting annotation updates and viewing its annotation at NCBI.
Also see our acknowledgements page