Pseudomonas aeruginosa PAO1, PA3769 (guaA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3769
Name
guaA
Replicon chromosome
Genomic location 4225660 - 4227237 (- strand)

Cross-References

RefSeq NP_252458.1
GI 15598964
Affymetrix PA3769_guaA_at
Entrez 880552
GenBank AAG07156.1
INSDC AAG07156.1
NCBI Locus Tag PA3769
protein_id(GenBank) gb|AAG07156.1|AE004796_1|gnl|PseudoCAP|PA3769
TIGR NTL03PA03769
UniParc UPI000012BD6A
UniProtKB Acc Q9HXM6
UniProtKB ID GUAA_PSEAE
UniRef100 UniRef100_B7UXJ9
UniRef50 UniRef50_O66601
UniRef90 UniRef90_Q02RS2

Product

Feature Type CDS
Coding Frame 1
Product Name
GMP synthase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -13.83
Kyte-Doolittle Hydrophobicity Value 0.003
Molecular Weight (kDa) 58.0
Isoelectric Point (pI) 5.57

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 652 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001894 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3769
Search term: guaA
Search term: GMP synthase

Human Homologs

Ensembl 110, assembly GRCh38.p14
guanine monophosphate synthase [Source:HGNC Symbol;Acc:HGNC:4378]
E-value: 2.3e-79
Percent Identity: 35.6

References

Nucleotide sequence of the guaA gene encoding GMP synthetase of Escherichia coli K12.
Tiedeman AA, Smith JM, Zalkin H
J Biol Chem 1985 Jul 25;260(15):8676-9
PubMed ID: 3894345
The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families.
Tesmer JJ, Klem TJ, Deras ML, Davisson VJ, Smith JL
Nat Struct Biol 1996 Jan;3(1):74-86
PubMed ID: 8548458
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994