Pseudomonas aeruginosa PAO1, PA3810 (hscA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3810
Name
hscA
Replicon chromosome
Genomic location 4267717 - 4269576 (- strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_252499.1
GI 15599005
Affymetrix PA3810_hscA_at
Entrez 879936
GenBank AAG07197.1
INSDC AAC44151.1
NCBI Locus Tag PA3810
protein_id(GenBank) gb|AAG07197.1|AE004799_3|gnl|PseudoCAP|PA3810
TIGR NTL03PA03810
UniParc UPI000012CCDA
UniProtKB Acc Q51382
UniProtKB ID HSCA_PSEAE
UniRef100 UniRef100_Q51382
UniRef50 UniRef50_Q8ZCS5
UniRef90 UniRef90_Q51382

Product

Feature Type CDS
Coding Frame 1
Product Name
heat shock protein HscA
Product Name Confidence: Class 1
Synonyms Hsc66
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -22.86
Kyte-Doolittle Hydrophobicity Value -0.100
Molecular Weight (kDa) 66.4
Isoelectric Point (pI) 4.61

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 637 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001856 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3810
Search term: hscA

Human Homologs

Ensembl 110, assembly GRCh38.p14
heat shock protein family A (Hsp70) member 9 [Source:HGNC Symbol;Acc:HGNC:5244]
E-value: 5.3e-112
Percent Identity: 40.4
Ensembl 110, assembly GRCh38.p14
heat shock protein family A (Hsp70) member 9 [Source:HGNC Symbol;Acc:HGNC:5244]
E-value: 5.3e-112
Percent Identity: 40.4

References

Mutations in a gene encoding a new Hsp70 suppress rapid DNA inversion and bgl activation, but not proU derepression, in hns-1 mutant Escherichia coli.
Kawula TH, Lelivelt MJ
J Bacteriol 1994 Feb;176(3):610-9
PubMed ID: 8300516
A gene encoding a DnaK/hsp70 homolog in Escherichia coli.
Seaton BL, Vickery LE
Proc Natl Acad Sci U S A 1994 Mar 15;91(6):2066-70
PubMed ID: 8134349
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994