Pseudomonas aeruginosa PAO1, PA3824 (queA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3824
Name
queA
Replicon chromosome
Genomic location 4280520 - 4281563 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252513.1
GI 15599019
Affymetrix PA3824_queA_at
DNASU PaCD00007666
Entrez 879887
GenBank AAG07211.1
INSDC AAG07211.1
NCBI Locus Tag PA3824
protein_id(GenBank) gb|AAG07211.1|AE004799_17|gnl|PseudoCAP|PA3824
TIGR NTL03PA03824
UniParc UPI0000132E8B
UniProtKB Acc Q9HXH8
UniProtKB ID QUEA_PSEAE
UniRef100 UniRef100_Q9HXH8
UniRef50 UniRef50_Q5P721
UniRef90 UniRef90_Q9HXH8

Product

Feature Type CDS
Coding Frame 1
Product Name
S-adenosylmethionine:trna ribosyltransferase-isomerase
Product Name Confidence: Class 2
Synonyms Queuosine biosynthesis protein QueA
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.89
Kyte-Doolittle Hydrophobicity Value -0.090
Molecular Weight (kDa) 38.2
Isoelectric Point (pI) 6.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 519 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001842 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3824
Search term: queA

Human Homologs

References

A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine.
Slany RK, Bösl M, Crain PF, Kersten H
Biochemistry 1993 Aug 3;32(30):7811-7
PubMed ID: 8347586