Pseudomonas aeruginosa PAO1, PA3859

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3859
Name
Replicon	chromosome
Genomic location	4318259 - 4318906 (+ strand)
Transposon Mutants	2 transposon mutants in PAO1
Transposon Mutants in orthologs	2 transposon mutants in orthologs
Comment	Optimal enzyme activity at 55 C and at pH 9.0 and is inhibited by phenylmethylsulfonyl fluoride. pI of 5.2 determined by IEF(theoretical pI of 5.0). Monomeric structure (24kDa).

Cross-References

RefSeq	NP_252548.1
GI	15599054
Affymetrix	PA3859_at
Entrez	879801
GenBank	AAG07246.1
INSDC	AAG07246.1
NCBI Locus Tag	PA3859
protein_id(GenBank)	gb|AAG07246.1|AE004803_2|gnl|PseudoCAP|PA3859
TIGR	NTL03PA03859
UniParc	UPI00000C5B47
UniProtKB Acc	Q9HXE7
UniProtKB ID	Q9HXE7_PSEAE
UniRef100	UniRef100_Q9HXE7
UniRef50	UniRef50_Q53547
UniRef90	UniRef90_Q9HXE7

Product

Feature Type	CDS
Coding Frame	1
Product Name	carboxylesterase Product Name Confidence: Class 1
Synonyms
Evidence for Translation	Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7)	-9.87
Kyte-Doolittle Hydrophobicity Value	0.017
Molecular Weight (kDa)	23.4
Isoelectric Point (pI)	4.87

Subcellular localization

Individual Mappings	Localization Confidence PMID Unknown Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
3CN9	HYDROLASE	03/25/08	Crystal Structure Analysis of the Carboxylesterase PA3859 from Pseudomonas aeruginosa PAO1- orthorhombic crystal form	Pseudomonas aeruginosa	2.09	X-RAY DIFFRACTION	100.0
3CN7	HYDROLASE	03/25/08	Crystal Structure Analysis of the Carboxylesterase PA3859 from Pseudomonas aeruginosa PAO1- MONOCLINIC CRYSTAL FORM	Pseudomonas aeruginosa	2.99	X-RAY DIFFRACTION	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 212 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG001810 (536 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3859 Search term: carboxylesterase

Human Homologs

Ensembl 110, assembly GRCh38.p14

ENSP00000363638 lysophospholipase 2 [Source:HGNC Symbol;Acc:HGNC:6738] E-value: 1.3e-25 Percent Identity: 34.8

References

Isolation, characterization, and heterologous expression of a carboxylesterase of Pseudomonas aeruginosa PAO1. Pesaresi A, Devescovi G, Lamba D, Venturi V, Degrassi G Curr Microbiol 2005 Feb;50(2):102-9 PubMed ID: 15717224

Characterization of Pseudomonas fluorescens carboxylesterase: cloning and expression of the esterase gene in Escherichia coli. Hong KH, Jang WH, Choi KD, Yoo OJ Agric Biol Chem 1991 Nov;55(11):2839-45 PubMed ID: 1368750

Crystal structure of carboxylesterase from Pseudomonas fluorescens, an alpha/beta hydrolase with broad substrate specificity. Kim KK, Song HK, Shin DH, Hwang KY, Choe S, Yoo OJ, Suh SW Structure 1997 Dec 15;5(12):1571-84 PubMed ID: 9438866