Pseudomonas aeruginosa PAO1, PA3924

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3924
Name
Replicon chromosome
Genomic location 4398259 - 4399941 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_252613.1
GI 15599119
Affymetrix PA3924_at
Entrez 879043
GenBank AAG07311.1
INSDC AAG07311.1
NCBI Locus Tag PA3924
protein_id(GenBank) gb|AAG07311.1|AE004810_3|gnl|PseudoCAP|PA3924
TIGR NTL03PA03924
UniParc UPI00000C5B7C
UniProtKB Acc Q9HX89
UniProtKB ID Q9HX89_PSEAE
UniRef100 UniRef100_W0YVX6
UniRef50 UniRef50_C0QAH4
UniRef90 UniRef90_A0A077JMM2

Product

Feature Type CDS
Coding Frame 1
Product Name
probable medium-chain acyl-CoA ligase
Product Name Confidence: Class 3
Synonyms probable acyl-CoA synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.21
Kyte-Doolittle Hydrophobicity Value -0.086
Molecular Weight (kDa) 62.3
Isoelectric Point (pI) 5.97

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 599 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001750 (523 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3924

Human Homologs

Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase bubblegum family member 2 [Source:HGNC Symbol;Acc:HGNC:24174]
E-value: 1.2e-09
Percent Identity: 20.9

References

DNA sequence determination and functional characterization of the OCT-plasmid-encoded alkJKL genes of Pseudomonas oleovorans.
van Beilen JB, Eggink G, Enequist H, Bos R, Witholt B
Mol Microbiol 1992 Nov;6(21):3121-36
PubMed ID: 1453953