Pseudomonas aeruginosa PAO1, PA3931

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3931
Name
Replicon chromosome
Genomic location 4406806 - 4407585 (+ strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 1XS5 (Lipoprotein Tp32 from Treponema pallidum, root-mean-square deviation: 0.6 angstroms). Sequence similarity to 4K3F (100%/72,38%) (MSE). Final predicted ligand: methionine (PMID: 31631799)

Cross-References

RefSeq NP_252620.1
GI 15599126
Affymetrix PA3931_at
DNASU PaCD00007715
Entrez 879071
GenBank AAG07318.1
INSDC AAG07318.1
NCBI Locus Tag PA3931
protein_id(GenBank) gb|AAG07318.1|AE004811_1|gnl|PseudoCAP|PA3931
TIGR NTL03PA03931
UniParc UPI00000C5B80
UniProtKB Acc Q9HX85
UniProtKB ID Q9HX85_PSEAE
UniRef100 UniRef100_Q9HX85
UniRef50 UniRef50_D3P6A1
UniRef90 UniRef90_Q9HX85

Product

Feature Type CDS
Coding Frame 1
Product Name
putative methionine-binding protein
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 1.48
Kyte-Doolittle Hydrophobicity Value -0.066
Molecular Weight (kDa) 28.1
Isoelectric Point (pI) 8.64

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown (This protein may have multiple localization sites) Class 3
Unknown (This protein may have multiple localization sites) Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 268 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG001744 (496 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3931

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Three contiguous lipoprotein genes in Pasteurella haemolytica A1 which are homologous to a lipoprotein gene in Haemophilus influenzae type b.
Cooney BJ, Lo RY
Infect Immun 1993 Nov;61(11):4682-8
PubMed ID: 8406866
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799