Pseudomonas aeruginosa PAO1, PA4430

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4430
Name
Replicon chromosome
Genomic location 4963055 - 4964266 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253120.1
GI 15599626
Affymetrix PA4430_at
Entrez 880944
GenBank AAG07818.1
INSDC AAG07818.1
NCBI Locus Tag PA4430
protein_id(GenBank) gb|AAG07818.1|AE004857_9|gnl|PseudoCAP|PA4430
TIGR NTL03PA04431
UniParc UPI00000C5D0B
UniProtKB Acc Q9HVY5
UniProtKB ID Q9HVY5_PSEAE
UniRef100 UniRef100_W0YYT9
UniRef50 UniRef50_Q4K6H5
UniRef90 UniRef90_A0A024HMV8

Product

Feature Type CDS
Coding Frame 1
Product Name
probable cytochrome b
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected using LC-MS/MS (46.07 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.85
Kyte-Doolittle Hydrophobicity Value 0.562
Molecular Weight (kDa) 46.1
Isoelectric Point (pI) 8.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 358 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004020 (517 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4430

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrially encoded cytochrome b [Source:HGNC Symbol;Acc:HGNC:7427]
E-value: 2.7e-72
Percent Identity: 42.3

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344