Pseudomonas aeruginosa PAO1, PA4566 (obg)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA4566
Name
obg
Replicon chromosome
Genomic location 5114670 - 5115890 (- strand)
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_253256.1
GI 15599762
Affymetrix PA4566_obg_at
Entrez 880877
GenBank AAG07954.1
INSDC AAG07954.1
NCBI Locus Tag PA4566
protein_id(GenBank) gb|AAG07954.1|AE004870_5|gnl|PseudoCAP|PA4566
TIGR NTL03PA04567
UniParc UPI00000C5D66
UniProtKB Acc Q9HVL8
UniProtKB ID OBG_PSEAE
UniRef100 UniRef100_B7V0A9
UniRef50 UniRef50_Q5ZS70
UniRef90 UniRef90_B7V0A9

Product

Feature Type CDS
Coding Frame 1
Product Name
GTP-binding protein Obg
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -19.03
Kyte-Doolittle Hydrophobicity Value -0.422
Molecular Weight (kDa) 44.3
Isoelectric Point (pI) 4.54

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 667 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG004225 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA4566
Search term: obg

Human Homologs

Ensembl 110, assembly GRCh38.p14
mitochondrial ribosome associated GTPase 2 [Source:HGNC Symbol;Acc:HGNC:16239]
E-value: 3.1e-52
Percent Identity: 41.8

References

A Vibrio harveyi insertional mutant in the cgtA (obg, yhbZ) gene, whose homologues are present in diverse organisms ranging from bacteria to humans and are essential genes in many bacterial species.
Czyz A, Zielke R, Konopa G, Wegrzyn G
Microbiology (Reading) 2001 Jan;147(Pt 1):183-91
PubMed ID: 11160812
Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
Fleischmann RD, Adams MD, White O, Clayton RA, Kirkness EF, Kerlavage AR, Bult CJ, Tomb JF, Dougherty BA, Merrick JM
Science 1995 Jul 28;269(5223):496-512
PubMed ID: 7542800
Obg, an essential GTP binding protein of Bacillus subtilis, is necessary for stress activation of transcription factor sigma(B).
Scott JM, Haldenwang WG
J Bacteriol 1999 Aug;181(15):4653-60
PubMed ID: 10419966