Pseudomonas aeruginosa PAO1, PA5049 (rpmE)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA5049
Name
rpmE
Replicon chromosome
Genomic location 5687105 - 5687320 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_253736.1
GI 15600242
Affymetrix PA5049_rpmE_at
DNASU PaCD00008171
Entrez 881149
GenBank AAG08434.1
INSDC AAG08434.1
NCBI Locus Tag PA5049
protein_id(GenBank) gb|AAG08434.1|AE004918_4|gnl|PseudoCAP|PA5049
TIGR NTL03PA05050
UniParc UPI0000133F21
UniProtKB Acc Q9HUD0
UniProtKB ID RL31_PSEAE
UniRef100 UniRef100_B7V3E2
UniRef50 UniRef50_Q7NX81
UniRef90 UniRef90_A6VDH0

Product

Feature Type CDS
Coding Frame 1
Product Name
50S ribosomal protein L31
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 4.55
Kyte-Doolittle Hydrophobicity Value -0.331
Molecular Weight (kDa) 7.9
Isoelectric Point (pI) 8.77

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7UNW RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.6 ELECTRON MICROSCOPY 100.0
7UNR RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0
7UNV RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.7 ELECTRON MICROSCOPY 100.0
7UNU RIBOSOME 04/11/22 Pseudomonas aeruginosa 70S ribosome initiation complex bound to compact IF2-GDP (composite structure I-B) Escherichia coli; Pseudomonas aeruginosa PAO1; SYNTHETIC CONSTRUCT 2.9 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 564 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002073 (927 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5049
Search term: rpmE

Human Homologs

References

Primary structure of Escherichia coli ribosomal protein L31.
Brosius J
Biochemistry 1978 Feb 7;17(3):501-8
PubMed ID: 339950
Modification of Escherichia coli ribosomes with the fluorescent reagent N-[[(iodoacetyl)amino]ethyl]-5-naphthylamine-1-sulfonic acid. Identification of derivatized L31' and studies on its intraribosomal properties.
Hanas JS, Simpson MV
Biochemistry 1985 Dec 3;24(25):7303-9
PubMed ID: 3910100