Pseudomonas aeruginosa PAO1, PA0148

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA0148
Name
Synonym: add
Replicon chromosome
Genomic location 168213 - 169163 (+ strand)
Transposon Mutants 6 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_248838.1
GI 15595346
Affymetrix PA0148_at
Entrez 879249
GenBank AAG03538.1
INSDC AAG03538.1
NCBI Locus Tag PA0148
protein_id(GenBank) gb|AAG03538.1|AE004453_1|gnl|PseudoCAP|PA0148
TIGR NTL03PA00149
UniParc UPI0000125510
UniProtKB Acc Q9I6Y4
UniProtKB ID ADE_PSEAE
UniRef100 UniRef100_B7V265
UniRef50 UniRef50_A6UXT7
UniRef90 UniRef90_A6UXT7

Product

Feature Type CDS
Coding Frame 1
Product Name
adenine deaminase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -14.43
Kyte-Doolittle Hydrophobicity Value -0.275
Molecular Weight (kDa) 36.2
Isoelectric Point (pI) 4.87

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
3OU8 HYDROLASE 09/14/10 The crystal structure of adenosine deaminase from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.51 X-RAY DIFFRACTION 99.7
3PBM HYDROLASE 10/20/10 The crystal structure of adenosine deaminase in complex with chloropurine from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.591 X-RAY DIFFRACTION 99.7
3PAO HYDROLASE 10/19/10 The crystal structure of adenosine deaminase with adenine bound from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.493 X-RAY DIFFRACTION 99.7
3PAN HYDROLASE 10/19/10 The crystal structure of adenosine deaminase with hypoxanthine bound from Pseudomonas aeruginosa Pseudomonas aeruginosa 2.627 X-RAY DIFFRACTION 99.7

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 254 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000146 (549 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0148
Search term: adenine deaminase

Human Homologs

Ensembl 107, assembly GRCh38.p13
adenosine deaminase [Source:HGNC Symbol;Acc:HGNC:186]
E-value: 1.7e-21
Percent Identity: 23.6

References

Deduced amino acid sequence of Escherichia coli adenosine deaminase reveals evolutionarily conserved amino acid residues: implications for catalytic function.
Chang ZY, Nygaard P, Chinault AC, Kellems RE
Biochemistry 1991 Feb 26;30(8):2273-80
PubMed ID: 1998686
Pa0148 from Pseudomonas aeruginosa catalyzes the deamination of adenine.
Goble AM, Zhang Z, Sauder JM, Burley SK, Swaminathan S, Raushel FM
Biochemistry 2011 Aug;50(30):6589-97
PubMed ID: 21710971