Pseudomonas aeruginosa PAO1, PA0285 (pipA)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0285
|
| Name |
pipA
|
| Replicon | chromosome |
| Genomic location | 318312 - 320594 (- strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_248976.1 |
| GI | 15595482 |
| Affymetrix | PA0285_at |
| Entrez | 879674 |
| GenBank | AAG03674.1 |
| INSDC | AAG03674.1 |
| NCBI Locus Tag | PA0285 |
| protein_id(GenBank) | gb|AAG03674.1|AE004466_8|gnl|PseudoCAP|PA0285 |
| TIGR | NTL03PA00286 |
| UniParc | UPI00000C4FE1 |
| UniProtKB Acc | Q9I6K5 |
| UniProtKB ID | Q9I6K5_PSEAE |
| UniRef100 | UniRef100_Q9I6K5 |
| UniRef50 | UniRef50_Q4KK97 |
| UniRef90 | UniRef90_Q9I6K5 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
cyclic di-GMP phosphodiesterase, PipA
|
| Synonyms |
putative phosphodiesterase |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
|
| Charge (pH 7) | -12.43 |
| Kyte-Doolittle Hydrophobicity Value | -0.275 |
| Molecular Weight (kDa) | 86.4 |
| Isoelectric Point (pI) | 6.31 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 536 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000282 (532 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0285
Search term: pipA
Search term: cyclic di-GMP phosphodiesterase, PipA
|
Human Homologs
References
|
Diguanylate Cyclases and Phosphodiesterases Required for Basal-Level c-di-GMP in <i>Pseudomonas aeruginosa</i> as Revealed by Systematic Phylogenetic and Transcriptomic Analyses.
Wei Q, Leclercq S, Bhasme P, Xu A, Zhu B, Zhang Y, Zhang M, Wang S, Ma LZ
Appl Environ Microbiol 2019 Nov 1;85(21)
PubMed ID: 31444209
|
|
The c-di-GMP Phosphodiesterase PipA (PA0285) Regulates Autoaggregation and Pf4 Bacteriophage Production in Pseudomonas aeruginosa PAO1.
Cai YM, Yu KW, Liu JH, Cai Z, Zhou ZH, Liu Y, Wang TF, Yang L
Appl Environ Microbiol 2022 Jun 28;88(12):e0003922
PubMed ID: 35638845
|