Pseudomonas aeruginosa PAO1, PA0763 (mucA)

Cytoplasmic Membrane
Outer Membrane
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 831914 - 832498 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1


RefSeq NP_249454.1
GI 15595960
Affymetrix PA0763_mucA_at
Entrez 879357
GenBank AAG04152.1
INSDC AAG04152.1
NCBI Locus Tag PA0763
protein_id(GenBank) gb|AAG04152.1|AE004511_5|gnl|PseudoCAP|PA0763
UniParc UPI000012F8D3
UniProtKB Acc P38107
UniRef100 UniRef100_P38107
UniRef50 UniRef50_P38107
UniRef90 UniRef90_P38107


Feature Type CDS
Coding Frame 1
Product Name
anti-sigma factor MucA
Product Name Confidence: Class 1
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.31
Kyte-Doolittle Hydrophobicity Value -0.315
Molecular Weight (kDa) 21.0
Isoelectric Point (pI) 5.74

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 1
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6IN7 TRANSCRIPTION 10/24/18 Crystal structure of AlgU in complex with MucA(cyto) PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.96 X-RAY DIFFRACTION 100.0
6IN9 MEMBRANE PROTEIN 10/24/18 Crystal structure of MucB in complex with MucA(peri) PSEUDOMONAS AERUGINOSA (STRAIN ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 1.801 X-RAY DIFFRACTION 100.0
6JAU PROTEIN BINDING 01/25/19 The complex structure of Pseudomonas aeruginosa MucA/MucB. PSEUDOMONAS AERUGINOSA (STRAIN PAO1) 1.905 X-RAY DIFFRACTION 100.0

Virulence Evidence

Source 1
Database Victors : 4227
Category Alginate regulation
Evidence ECO:0000015
mutant phenotype evidence
Host Organism Medicago sativa
Notes mucA mutant attenuated for virulence in alfalfa seedling Infection model
Reference 12426404
Source 2
Database VFDB : VF0091
Category Alginate regulation
Host Organism

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 45 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000737 (510 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0763
Search term: mucA

Human Homologs


Posttranslational control of the algT (algU)-encoded sigma22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN).
Mathee K, McPherson CJ, Ohman DE
J Bacteriol 1997 Jun;179(11):3711-20
PubMed ID: 9171421
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients.
Martin DW, Schurr MJ, Mudd MH, Govan JR, Holloway BW, Deretic V
Proc. Natl. Acad. Sci. U.S.A. 1993 Sep;90(18):8377-81
PubMed ID: 8378309
Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa.
Qiu D, Eisinger VM, Rowen DW, Yu HD
Proc. Natl. Acad. Sci. U.S.A. 2007 May;104(19):8107-12
PubMed ID: 17470813
The Pseudomonas aeruginosa sensor kinase KinB negatively controls alginate production through AlgW-dependent MucA proteolysis.
Damron FH, Qiu D, Yu HD
J. Bacteriol. 2009 Apr;191(7):2285-95
PubMed ID: 19168621
Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy.
Schurr MJ, Martin DW, Mudd MH, Deretic V
J. Bacteriol. 1994 Jun;176(11):3375-82
PubMed ID: 8195094
Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA.
Xie ZD, Hershberger CD, Shankar S, Ye RW, Chakrabarty AM
J. Bacteriol. 1996 Aug;178(16):4990-6
PubMed ID: 8759865
Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control.
Fyfe JA, Govan JR
J. Gen. Microbiol. 1980 Aug;119(2):443-50
PubMed ID: 6785378
Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate.
Ohman DE, Chakrabarty AM
Infect. Immun. 1981 Jul;33(1):142-8
PubMed ID: 6790439