Pseudomonas aeruginosa PAO1, PA3098 (xcpW)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3098
Name
xcpW
Synonym: pddD
Replicon chromosome
Genomic location 3478625 - 3479338 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251788.1
GI 15598294
Affymetrix PA3098_xcpW_at
DNASU PaCD00007363
Entrez 882776
GenBank AAG06486.1
INSDC AAG06486.1
NCBI Locus Tag PA3098
protein_id(GenBank) gb|AAG06486.1|AE004734_4|gnl|PseudoCAP|PA3098
TIGR NTL03PA03098
UniParc UPI000012BC5A
UniProtKB Acc Q00517
UniProtKB ID GSPJ_PSEAE
UniRef100 UniRef100_Q00517
UniRef50 UniRef50_Q00517
UniRef90 UniRef90_Q00517

Product

Feature Type CDS
Coding Frame 1
Product Name
general secretion pathway protein J
Product Name Confidence: Class 1
Synonyms PilD-dependent protein PddD
Evidence for Translation
Charge (pH 7) -6.03
Kyte-Doolittle Hydrophobicity Value -0.685
Molecular Weight (kDa) 27.1
Isoelectric Point (pI) 5.08

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5VTM TRANSPORT PROTEIN 05/17/17 The crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.041 X-RAY DIFFRACTION 100.0
3NJE PROTEIN TRANSPORT 06/17/10 Structure of the Minor Pseudopilin XcpW from the Pseudomonas aeruginosa Type II Secretion System Pseudomonas aeruginosa 1.85 X-RAY DIFFRACTION 100.0
5BW0 PROTEIN TRANSPORT 06/05/15 The crystal structure of minor pseudopilin binary complex of XcpV and XcpW from the Type 2 secretion system of Pseudomonas aeruginosa Pseudomonas aeruginosa 2 X-RAY DIFFRACTION 100.0
6UTU PROTEIN TRANSPORT 10/30/19 Crystal structure of minor pseudopilin ternary complex of XcpVWX from the Type 2 secretion system of Pseudomonas aeruginosa in the P3 space group Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.85 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 72 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000656 (743 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3098
Search term: xcpW

Human Homologs

References

Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W.
Nunn DN, Lory S
J Bacteriol 1993 Jul;175(14):4375-82
PubMed ID: 8331069
Components of the protein-excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase.
Nunn DN, Lory S
Proc Natl Acad Sci U S A 1992 Jan 1;89(1):47-51
PubMed ID: 1309616
Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase.
Bally M, Filloux A, Akrim M, Ball G, Lazdunski A, Tommassen J
Mol Microbiol 1992 May;6(9):1121-31
PubMed ID: 1588814