Pseudomonas aeruginosa PAO1, PA5479 (gltP)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest
Locus Tag
PA5479
Name
gltP
Replicon chromosome
Genomic location 6170176 - 6171510 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_254166.1
GI 15600672
Affymetrix PA5479_gltP_at
Entrez 877684
GenBank AAG08864.1
INSDC AAG08864.1
NCBI Locus Tag PA5479
protein_id(GenBank) gb|AAG08864.1|AE004960_7|gnl|PseudoCAP|PA5479
TIGR NTL03PA05480
UniParc UPI00000C602C
UniProtKB Acc Q9HT91
UniProtKB ID Q9HT91_PSEAE
UniRef100 UniRef100_W0YZQ9
UniRef50 UniRef50_P21345
UniRef90 UniRef90_A6VEV3

Product

Feature Type CDS
Coding Frame 1
Product Name
proton-glutamate symporter
Product Name Confidence: Class 2
Synonyms glutamate-aspartate symporter
Evidence for Translation
Detected using LC-MS/MS (47.62 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 6.19
Kyte-Doolittle Hydrophobicity Value 0.851
Molecular Weight (kDa) 47.6
Isoelectric Point (pI) 9.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Membrane Class 3
Cytoplasmic Membrane Class 2
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 435 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG005020 (533 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA5479
Search term: gltP

Human Homologs

Ensembl 97, assembly GRCh38.p12
solute carrier family 1 member 1 [Source:HGNC Symbol;Acc:HGNC:10939]
E-value: 1.4e-33
Percent Identity: 28.9

References

Cloning and sequencing of a gene encoding a glutamate and aspartate carrier of Escherichia coli K-12.
Wallace B, Yang YJ, Hong JS, Lum D
J Bacteriol 1990 Jun;172(6):3214-20
PubMed ID: 1971622
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344