Pseudomonas aeruginosa PAO1, PA0020 (tsaP)
Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
Gene Feature Overview
| Strain |
Pseudomonas aeruginosa PAO1 (Reference)
GCF_000006765.1|latest |
| Locus Tag |
PA0020
|
| Name |
tsaP
|
| Replicon | chromosome |
| Genomic location | 21712 - 22737 (+ strand) |
| Transposon Mutants | 2 transposon mutants in PAO1 |
| Transposon Mutants in orthologs | 1 transposon mutants in orthologs |
Cross-References
| RefSeq | NP_248710.1 |
| GI | 15595218 |
| Affymetrix | PA0020_at |
| DNASU | PaCD00006012 |
| Entrez | 879309 |
| GenBank | AAG03410.1 |
| INSDC | AAG03410.1 |
| NCBI Locus Tag | PA0020 |
| protein_id(GenBank) | gb|AAG03410.1|AE004441_11|gnl|PseudoCAP|PA0020 |
| TIGR | NTL03PA00021 |
| UniParc | UPI00000C4EED |
| UniProtKB Acc | Q9I7A7 |
| UniProtKB ID | Q9I7A7_PSEAE |
| UniRef100 | UniRef100_W0YJF2 |
| UniRef50 | UniRef50_A6UX82 |
| UniRef90 | UniRef90_A6UX82 |
Product
| Feature Type | CDS |
| Coding Frame | 1 |
| Product Name |
T4P secretin-associated protein TsaP
|
| Synonyms | |
| Evidence for Translation |
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
|
| Charge (pH 7) | 3.73 |
| Kyte-Doolittle Hydrophobicity Value | -0.248 |
| Molecular Weight (kDa) | 37.6 |
| Isoelectric Point (pI) | 9.37 |
Subcellular localization
| Individual Mappings | |
| Additional evidence for subcellular localization |
AlphaFold Protein Structure Database
Available for P. aeruginosa PAO1.
| View predicted 3D structure in AlphaFold DB |
PDB 3D Structures
| Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
| 6VE2 | PROTEIN TRANSPORT | 12/28/19 | Tetradecameric PilQ bound by TsaP heptamer from Pseudomonas aeruginosa | Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) | 4.3 | ELECTRON MICROSCOPY | 100.0 |
Pathogen Association Analysis
| Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 108 genera
|
Orthologs/Comparative Genomics
| Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
| Pseudomonas Ortholog Group |
POG000020 (480 members) |
| Putative Inparalogs | None Found |
Interactions
| STRING database | Search for predicted protein-protein interactions using:
Search term: PA0020
Search term: tsaP
Search term: T4P secretin-associated protein TsaP
|
Human Homologs
References
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
|
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
|
|
Peptidoglycan-binding protein TsaP functions in surface assembly of type IV pili.
Siewering K, Jain S, Friedrich C, Webber-Birungi MT, Semchonok DA, Binzen I, Wagner A, Huntley S, Kahnt J, Klingl A, Boekema EJ, Søgaard-Andersen L, van der Does C
Proc. Natl. Acad. Sci. U.S.A. 2014 Mar;111(10):E953-61
PubMed ID: 24556993
|