Pseudomonas aeruginosa PAO1, PA0020 (tsaP)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
Replicon chromosome
Genomic location 21712 - 22737 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_248710.1
GI 15595218
Affymetrix PA0020_at
DNASU PaCD00006012
Entrez 879309
GenBank AAG03410.1
INSDC AAG03410.1
NCBI Locus Tag PA0020
protein_id(GenBank) gb|AAG03410.1|AE004441_11|gnl|PseudoCAP|PA0020
UniParc UPI00000C4EED
UniProtKB Acc Q9I7A7
UniRef100 UniRef100_W0YJF2
UniRef50 UniRef50_A6UX82
UniRef90 UniRef90_A6UX82


Feature Type CDS
Coding Frame 1
Product Name
T4P secretin-associated protein TsaP
Product Name Confidence: Class 2
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 3.73
Kyte-Doolittle Hydrophobicity Value -0.248
Molecular Weight (kDa) 37.6
Isoelectric Point (pI) 9.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold Protein Structure Database

Available for P. aeruginosa PAO1.

View predicted 3D structure in AlphaFold DB

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6VE2 PROTEIN TRANSPORT 12/28/19 Tetradecameric PilQ bound by TsaP heptamer from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 4.3 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 108 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000020 (480 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0020
Search term: tsaP

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Peptidoglycan-binding protein TsaP functions in surface assembly of type IV pili.
Siewering K, Jain S, Friedrich C, Webber-Birungi MT, Semchonok DA, Binzen I, Wagner A, Huntley S, Kahnt J, Klingl A, Boekema EJ, Søgaard-Andersen L, van der Does C
Proc. Natl. Acad. Sci. U.S.A. 2014 Mar;111(10):E953-61
PubMed ID: 24556993