Pseudomonas aeruginosa PAO1, PA0020 (tsaP)

Cytoplasmic Membrane
Outer Membrane
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
Locus Tag
Replicon chromosome
Genomic location 21712 - 22737 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs


RefSeq NP_248710.1
GI 15595218
Affymetrix PA0020_at
DNASU PaCD00006012
Entrez 879309
GenBank AAG03410.1
INSDC AAG03410.1
NCBI Locus Tag PA0020
protein_id(GenBank) gb|AAG03410.1|AE004441_11|gnl|PseudoCAP|PA0020
UniParc UPI00000C4EED
UniProtKB Acc Q9I7A7
UniRef100 UniRef100_W0YJF2
UniRef50 UniRef50_A6UX82
UniRef90 UniRef90_A6UX82


Feature Type CDS
Coding Frame 1
Product Name
T4P secretin-associated protein TsaP
Product Name Confidence: Class 2
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 3.73
Kyte-Doolittle Hydrophobicity Value -0.248
Molecular Weight (kDa) 37.6
Isoelectric Point (pI) 9.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
6VE2 PROTEIN TRANSPORT 12/28/19 Tetradecameric PilQ bound by TsaP heptamer from Pseudomonas aeruginosa Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 4.3 ELECTRON MICROSCOPY 100.0

Pathogen Association Analysis

Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 108 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000020 (467 members)
Putative Inparalogs None Found


STRING database Search for predicted protein-protein interactions using:
Search term: PA0020
Search term: tsaP

Human Homologs


Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Peptidoglycan-binding protein TsaP functions in surface assembly of type IV pili.
Siewering K, Jain S, Friedrich C, Webber-Birungi MT, Semchonok DA, Binzen I, Wagner A, Huntley S, Kahnt J, Klingl A, Boekema EJ, Søgaard-Andersen L, van der Does C
Proc Natl Acad Sci U S A 2014 Mar 11;111(10):E953-61
PubMed ID: 24556993