Pseudomonas aeruginosa PAO1, PA0430 (metF)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0430
Name
metF
Replicon chromosome
Genomic location 481196 - 482068 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_249121.1
GI 15595627
Affymetrix PA0430_metF_at
DNASU PaCD00006182
Entrez 877765
GenBank AAG03819.1
INSDC AAG03819.1
NCBI Locus Tag PA0430
protein_id(GenBank) gb|AAG03819.1|AE004480_3|gnl|PseudoCAP|PA0430
TIGR NTL03PA00431
UniParc UPI00000C504E
UniProtKB Acc Q9I687
UniProtKB ID Q9I687_PSEAE
UniRef100 UniRef100_W0YKH2
UniRef50 UniRef50_G2DES0
UniRef90 UniRef90_A6UYP0

Product

Feature Type CDS
Coding Frame 1
Product Name
5,10-methylenetetrahydrofolate reductase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.68
Kyte-Doolittle Hydrophobicity Value -0.236
Molecular Weight (kDa) 32.2
Isoelectric Point (pI) 6.52

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 397 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000421 (552 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0430
Search term: metF

References

Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.
Saint-Girons I, Duchange N, Zakin MM, Park I, Margarita D, Ferrara P, Cohen GN
Nucleic Acids Res. 1983 Oct;11(19):6723-32
PubMed ID: 6356036
Operator-constitutive mutations of the Escherichia coli metF gene.
Belfaiza J, Guillou Y, Margarita D, Perrin D, Saint Girons I
J. Bacteriol. 1987 Feb;169(2):670-4
PubMed ID: 3542965
Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli.
Sheppard CA, Trimmer EE, Matthews RG
J. Bacteriol. 1999 Feb;181(3):718-25
PubMed ID: 9922232
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994