Pseudomonas aeruginosa PAO1, PA0552 (pgk)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse View in GBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0552
Name
pgk
Replicon chromosome
Genomic location 611281 - 612444 (+ strand)
Transposon Mutants 1 transposon mutants in PAO1

Cross-References

RefSeq NP_249243.1
GI 15595749
Affymetrix PA0552_pgk_at
Entrez 879198
GenBank AAG03941.1
INSDC AAG03941.1
NCBI Locus Tag PA0552
protein_id(GenBank) gb|AAG03941.1|AE004491_8|gnl|PseudoCAP|PA0552
TIGR NTL03PA00553
UniParc UPI0000131808
UniProtKB Acc Q9I5Y4
UniProtKB ID PGK_PSEAE
UniRef100 UniRef100_Q02TL3
UniRef50 UniRef50_P43726
UniRef90 UniRef90_Q1I3Y0

Product

Feature Type CDS
Coding Frame 1
Product Name
phosphoglycerate kinase
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -7.11
Kyte-Doolittle Hydrophobicity Value 0.206
Molecular Weight (kDa) 40.4
Isoelectric Point (pI) 5.06

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

Virulence Evidence

Source 1
Database Victors : 4225
Category Not available / Not known
Evidence ECO:0000315
mutant phenotype evidence used in manual assertion
Host Organism Rattus norvegicus
Notes
Infection model Rat agar bead model of chronic lung infection
Reference 14641575

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 664 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000536 (553 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0552
Search term: pgk

Human Homologs

Ensembl 94, assembly GRCh38.p12
phosphoglycerate kinase 1 [Source:HGNC Symbol;Acc:HGNC:8896]
E-value: 6.2e-67
Percent Identity: 39.2

References

Cloning and characterization of the Escherichia coli phosphoglycerate kinase (pgk) gene.
Nellemann LJ, Holm F, Atlung T, Hansen FG
Gene 1989 Apr;77(1):185-91
PubMed ID: 2545537
Identification, molecular cloning and sequence analysis of a gene cluster encoding the class II fructose 1,6-bisphosphate aldolase, 3-phosphoglycerate kinase and a putative second glyceraldehyde 3-phosphate dehydrogenase of Escherichia coli.
Alefounder PR, Perham RN
Mol. Microbiol. 1989 Jun;3(6):723-32
PubMed ID: 2546007
Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium Thermotoga maritima.
Auerbach G, Jacob U, Grättinger M, Schurig H, Jaenicke R
Biol. Chem. ;378(3-4):327-9
PubMed ID: 9165089
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994