Pseudomonas aeruginosa PAO1, PA0745 (dspI)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0745
Name
dspI
Synonym: dispersion inducer
Replicon chromosome
Genomic location 812445 - 813263 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs
Comment

Cross-References

RefSeq NP_249436.1
GI 15595942
Affymetrix PA0745_at
Entrez 878156
GenBank AAG04134.1
INSDC AAG04134.1
NCBI Locus Tag PA0745
protein_id(GenBank) gb|AAG04134.1|AE004509_9|gnl|PseudoCAP|PA0745
TIGR NTL03PA00746
UniParc UPI00000C5134
UniProtKB Acc Q9I5I4
UniProtKB ID Q9I5I4_PSEAE
UniRef100 UniRef100_W0YNS2
UniRef50 UniRef50_A9ANK2
UniRef90 UniRef90_W6RIF3

Product

Feature Type CDS
Coding Frame 1
Product Name
DspI
Product Name Confidence: Class 1
Synonyms probable enoyl-CoA hydratase/isomerase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -1.94
Kyte-Doolittle Hydrophobicity Value -0.216
Molecular Weight (kDa) 29.9
Isoelectric Point (pI) 6.37

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5WYD ISOMERASE 01/12/17 Structural of Pseudomonas aeruginosa DspI PSEUDOMONAS AERUGINOSA PAO1 2.101 X-RAY DIFFRACTION 99.6
5WYB ISOMERASE 01/12/17 Structure of Pseudomonas aeruginosa DspI PSEUDOMONAS AERUGINOSA PA14 2.25 X-RAY DIFFRACTION 99.6

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 508 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000719 (1195 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0745
Search term: dspI
Search term: DspI

Human Homologs

Ensembl 94, assembly GRCh38.p12
AU RNA binding methylglutaconyl-CoA hydratase [Source:HGNC Symbol;Acc:HGNC:890]
E-value: 7.5e-27
Percent Identity: 32.1

References

PubMed ID: 29500457
Genome-wide identification of Pseudomonas aeruginosa virulence-related genes using a Caenorhabditis elegans infection model.
Feinbaum RL, Urbach JM, Liberati NT, Djonovic S, Adonizio A, Carvunis AR, Ausubel FM
PLoS Pathog. 2012 ;8(7):e1002813
PubMed ID: 22911607