Pseudomonas aeruginosa PAO1, PA0928 (gacS)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
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Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Reference)
Locus Tag
PA0928
Name
gacS
Synonym: lemA
Replicon chromosome
Genomic location 1012975 - 1015752 (- strand)
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_249619.1
GI 15596125
Affymetrix PA0928_at
Entrez 880882
GenBank AAG04317.1
INSDC AAG04317.1
NCBI Locus Tag PA0928
protein_id(GenBank) gb|AAG04317.1|AE004527_4|gnl|PseudoCAP|PA0928
TIGR NTL03PA00929
UniParc UPI00000D428B
UniProtKB Acc G3XD98
UniProtKB ID G3XD98_PSEAE
UniRef100 UniRef100_G3XD98
UniRef50 UniRef50_P48027
UniRef90 UniRef90_G3XD98

Product

Feature Type CDS
Coding Frame 1
Product Name
sensor/response regulator hybrid
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -35.52
Kyte-Doolittle Hydrophobicity Value -0.160
Molecular Weight (kDa) 101.0
Isoelectric Point (pI) 4.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Additional evidence for subcellular localization

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
5O7J SIGNALING PROTEIN 06/09/17 Structural insights into the periplasmic sensor domain of the GacS histidine kinase controlling biofilm formation in Pseudomonas aeruginosa PSEUDOMONAS AERUGINOSA NOT SOLUTION NMR 100.0

Virulence Evidence

Source 1
Database VFDB
Category GacS/GacA two-component system
Evidence
Host Organism
Notes
Reference

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 624 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG000894 (544 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA0928
Search term: gacS

References

A signaling network reciprocally regulates genes associated with acute infection and chronic persistence in Pseudomonas aeruginosa.
Goodman AL, Kulasekara B, Rietsch A, Boyd D, Smith RS, Lory S
Dev. Cell 2004 Nov;7(5):745-54
PubMed ID: 15525535
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res. 2005 Feb;15(2):321-9
PubMed ID: 15687295
A global regulator of secondary metabolite production in Pseudomonas fluorescens Pf-5.
Corbell N, Loper JE
J. Bacteriol. 1995 Nov;177(21):6230-6
PubMed ID: 7592389