Pseudomonas aeruginosa PAO1, PA2619 (infA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2619
Name
infA
Replicon chromosome
Genomic location 2962003 - 2962221 (+ strand)

Cross-References

RefSeq NP_251309.1
GI 15597815
Affymetrix PA2619_infA_at
DNASU PaCD00007130
Entrez 882325
GenBank AAG06007.1
INSDC AAG06007.1
NCBI Locus Tag PA2619
protein_id(GenBank) gb|AAG06007.1|AE004690_7|gnl|PseudoCAP|PA2619
TIGR NTL03PA02619
UniParc UPI000000F8CE
UniProtKB Acc P65116
UniProtKB ID IF1_PSEAE
UniRef100 UniRef100_Q48H67
UniRef50 UniRef50_Q48H67
UniRef90 UniRef90_Q48H67

Product

Feature Type CDS
Coding Frame 1
Product Name
initiation factor
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.46
Kyte-Doolittle Hydrophobicity Value -0.512
Molecular Weight (kDa) 8.3
Isoelectric Point (pI) 9.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
2N78 TRANSLATION 09/04/15 NMR structure of IF1 from Pseudomonas aeruginosa Pseudomonas aeruginosa NOT SOLUTION NMR 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 590 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002933 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2619
Search term: infA
Search term: initiation factor

Human Homologs

References

Cloning and mapping of infA, the gene for protein synthesis initiation factor IF1.
Sands JF, Cummings HS, Sacerdot C, Dondon L, Grunberg-Manago M, Hershey JW
Nucleic Acids Res 1987 Jul 10;15(13):5157-68
PubMed ID: 3037488
The structure of the translational initiation factor IF1 from E.coli contains an oligomer-binding motif.
Sette M, van Tilborg P, Spurio R, Kaptein R, Paci M, Gualerzi CO, Boelens R
EMBO J 1997 Mar 17;16(6):1436-43
PubMed ID: 9135158
The complete genome sequence of Escherichia coli K-12.
Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y
Science 1997 Sep 5;277(5331):1453-62
PubMed ID: 9278503
Initiation factors IF1 and IF2 synergistically remove peptidyl-tRNAs with short polypeptides from the P-site of translating Escherichia coli ribosomes.
Karimi R, Pavlov MY, Heurgué-Hamard V, Buckingham RH, Ehrenberg M
J Mol Biol 1998 Aug 14;281(2):241-52
PubMed ID: 9698545