Strain |
Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest |
Locus Tag |
PA2934
|
Name |
cif
|
Replicon | chromosome |
Genomic location | 3289715 - 3290674 (+ strand) |
Transposon Mutants | 2 transposon mutants in PAO1 |
Transposon Mutants in orthologs | 7 transposon mutants in orthologs |
RefSeq | NP_251624.1 |
GI | 15598130 |
Affymetrix | PA2934_at |
DNASU | PaCD00007278 |
Entrez | 882654 |
GenBank | AAG06322.1 |
INSDC | AAG06322.1 |
NCBI Locus Tag | PA2934 |
protein_id(GenBank) | gb|AAG06322.1|AE004719_8|gnl|PseudoCAP|PA2934 |
TIGR | NTL03PA02934 |
UniParc | UPI00000C5891 |
UniProtKB Acc | Q9HZR3 |
UniProtKB ID | Q9HZR3_PSEAE |
UniRef100 | UniRef100_Q9HZR3 |
UniRef50 | UniRef50_Q9HZR3 |
UniRef90 | UniRef90_Q9HZR3 |
Feature Type | CDS |
Coding Frame | 1 |
Product Name |
CFTR inhibitory factor, Cif
|
Synonyms | |
Evidence for Translation | |
Charge (pH 7) | -3.21 |
Kyte-Doolittle Hydrophobicity Value | -0.157 |
Molecular Weight (kDa) | 35.8 |
Isoelectric Point (pI) | 6.51 |
Individual Mappings | |
Additional evidence for subcellular localization |
Accession | Header | Accession Date | Compound | Source | Resolution | Method | Percent Identity |
5HK9 | HYDROLASE/HYDROLASE INHIBITOR | 01/13/16 | Crystal structure of the CFTR inhibitory factor Cif bound to a urea inhibitor | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.8 | X-RAY DIFFRACTION | 99.3 |
4EUS | HYDROLASE | 04/25/12 | Crystal structure of the CFTR inhibitory factor Cif bound to 1,2-hexanediol | Pseudomonas aeruginosa | 1.65 | X-RAY DIFFRACTION | 99.3 |
5HKA | Hydrolase/Hydrolase inhibitor | 01/13/16 | Crystal structure of the CFTR inhibitory factor Cif bound to an amide inhibitor | Pseudomonas aeruginosa (strain UCBPP-PA14) | 2.05 | X-RAY DIFFRACTION | 99.3 |
4YX9 | Hydrolase/Hydrolase inhibitor | 03/22/15 | Crystal structure of the CFTR inhibitory factor Cif bound to tiratricol | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.75 | X-RAY DIFFRACTION | 99.3 |
3KD2 | HYDROLASE | 10/22/09 | Crystal structure of the CFTR inhibitory factor Cif | Pseudomonas aeruginosa UCBPP-PA14 | 1.8 | X-RAY DIFFRACTION | 99.3 |
5HKB | Hydrolase/Hydrolase inhibitor | 01/13/16 | Crystal structure of the CFTR inhibitory factor Cif bound to the inhibitor KB2115 | Pseudomonas aeruginosa UCBPP-PA14 | 1.65 | X-RAY DIFFRACTION | 99.3 |
5TNM | HYDROLASE | 10/14/16 | Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted (R)-1,2-Epoxyoctane hydrolysis intermediate | Pseudomonas aeruginosa (strain UCBPP-PA14) | 1.7 | X-RAY DIFFRACTION | 99.0 |
Source 1 | |
Database | PseudoCAP |
Category | |
Evidence |
ECO:0000314
direct assay evidence used in manual assertion
|
Host Organism | Homo sapiens |
Notes | |
Infection model | Human airway epithelial cells (Calu-3) |
Reference | 16236828 |
Results |
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 302 genera
|
Pseudomonas Ortholog Database | View orthologs at Pseudomonas Ortholog Database |
Pseudomonas Ortholog Group |
POG000798 (2691 members) |
Putative Inparalogs | None Found |
STRING database | Search for predicted protein-protein interactions using:
Search term: PA2934
Search term: cif
Search term: CFTR inhibitory factor, Cif
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Ensembl
110, assembly
GRCh38.p14
|
mesoderm specific transcript [Source:HGNC Symbol;Acc:HGNC:7028]
E-value:
8.5e-05
Percent Identity:
32.5
|
Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
|
Cif is negatively regulated by the TetR family repressor CifR.
MacEachran DP, Stanton BA, O'Toole GA
Infect Immun 2008 Jul;76(7):3197-206
PubMed ID: 18458065
|
The Pseudomonas aeruginosa secreted protein PA2934 decreases apical membrane expression of the cystic fibrosis transmembrane conductance regulator.
MacEachran DP, Ye S, Bomberger JM, Hogan DA, Swiatecka-Urban A, Stanton BA, O'Toole GA
Infect Immun 2007 Aug;75(8):3902-12
PubMed ID: 17502391
|
Pseudomonas aeruginosa inhibits endocytic recycling of CFTR in polarized human airway epithelial cells.
Swiatecka-Urban A, Moreau-Marquis S, Maceachran DP, Connolly JP, Stanton CR, Su JR, Barnaby R, O'toole GA, Stanton BA
Am J Physiol Cell Physiol 2006 Mar;290(3):C862-72
PubMed ID: 16236828
|
Chemotoxicity of doxorubicin and surface expression of P-glycoprotein (MDR1) is regulated by the Pseudomonas aeruginosa toxin Cif.
Ye S, MacEachran DP, Hamilton JW, O'Toole GA, Stanton BA
Am J Physiol Cell Physiol 2008 Sep;295(3):C807-18
PubMed ID: 18650266
|
Purification, crystallization and preliminary X-ray diffraction analysis of Cif, a virulence factor secreted by Pseudomonas aeruginosa.
Bahl CD, MacEachran DP, O'Toole GA, Madden DR
Acta Crystallogr Sect F Struct Biol Cryst Commun 2010 Jan 1;66(Pt 1):26-8
PubMed ID: 20057063
|