Pseudomonas aeruginosa PAO1, PA2999 (nqrA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA2999
Name
nqrA
Replicon chromosome
Genomic location 3356162 - 3357499 (- strand)
Transposon Mutants 3 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251689.1
GI 15598195
Affymetrix PA2999_nqrA_at
Entrez 880558
GenBank AAG06387.1
INSDC AAG06387.1
NCBI Locus Tag PA2999
protein_id(GenBank) gb|AAG06387.1|AE004724_16|gnl|PseudoCAP|PA2999
TIGR NTL03PA02999
UniParc UPI0000130434
UniProtKB Acc Q9HZK6
UniProtKB ID NQRA_PSEAE
UniRef100 UniRef100_Q9HZK6
UniRef50 UniRef50_Q8ZBZ0
UniRef90 UniRef90_Q9HZK6

Product

Feature Type CDS
Coding Frame 1
Product Name
Na+-translocating NADH:ubiquinone oxidoreductase subunit Nrq1
Product Name Confidence: Class 2
Synonyms Na+-translocating NADH:ubiquinone oxidoreductase alpha subunit
Evidence for Translation
Detected using LC-MS/MS (48.08 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 2.05
Kyte-Doolittle Hydrophobicity Value -0.040
Molecular Weight (kDa) 48.1
Isoelectric Point (pI) 7.63

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 120 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002582 (339 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA2999
Search term: nqrA

Human Homologs

References

Cloning of the Na(+)-translocating NADH-quinone reductase gene from the marine bacterium Vibrio alginolyticus and the expression of the beta-subunit in Escherichia coli.
Hayashi M, Hirai K, Unemoto T
FEBS Lett 1994 Dec 19;356(2-3):330-2
PubMed ID: 7805866
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344