Pseudomonas aeruginosa PAO1, PA3054

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3054
Name
Replicon chromosome
Genomic location 3418288 - 3420192 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs
CommentStructural similarity to 1H8L: human carboxypeptidase M (root-mean-square deviation: 2.2 angstroms)

Cross-References

RefSeq NP_251744.1
GI 15598250
Affymetrix PA3054_at
Entrez 882863
GenBank AAG06442.1
INSDC AAG06442.1
NCBI Locus Tag PA3054
protein_id(GenBank) gb|AAG06442.1|AE004730_6|gnl|PseudoCAP|PA3054
TIGR NTL03PA03054
UniParc UPI00000C58EE
UniProtKB Acc Q9HZF4
UniProtKB ID Q9HZF4_PSEAE
UniRef100 UniRef100_Q9HZF4
UniRef50 UniRef50_Q9HZF4
UniRef90 UniRef90_Q9HZF4

Product

Feature Type CDS
Coding Frame 1
Product Name
putative carboxypeptidase
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -2.94
Kyte-Doolittle Hydrophobicity Value -0.315
Molecular Weight (kDa) 71.2
Isoelectric Point (pI) 6.59

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 1
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 66 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002535 (308 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3054

Human Homologs

References

Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Uncovering the structure and function of Pseudomonas aeruginosa periplasmic proteins by an in silico approach.
Caprari S, Brandi V, Pasquadibisceglie A, Polticelli F
J Biomol Struct Dyn 2020 Sep;38(15):4508-4520
PubMed ID: 31631799