Pseudomonas aeruginosa PAO1, PA3086 (glpG)

Gene Feature Overview

Strain	Pseudomonas aeruginosa PAO1 (Stover et al., 2000) GCF_000006765.1|latest
Locus Tag	PA3086
Name	glpG
Replicon	chromosome
Genomic location	3464236 - 3465096 (- strand)
Transposon Mutants	1 transposon mutants in PAO1
Transposon Mutants in orthologs	1 transposon mutants in orthologs

Cross-References

RefSeq	NP_251776.1
GI	15598282
Affymetrix	PA3086_at
Entrez	882728
GenBank	AAG06474.1
INSDC	AAG06474.1
NCBI Locus Tag	PA3086
protein_id(GenBank)	gb|AAG06474.1|AE004733_1|gnl|PseudoCAP|PA3086
TIGR	NTL03PA03086
UniParc	UPI00000C590D
UniProtKB Acc	Q9HZC2
UniProtKB ID	Q9HZC2_PSEAE
UniRef100	UniRef100_W0YWE2
UniRef50	UniRef50_W6QZ77
UniRef90	UniRef90_A6V2Z0

Product

Feature Type	CDS
Coding Frame	1
Product Name	rhomboid family protease GlpG Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Charge (pH 7)	9.10
Kyte-Doolittle Hydrophobicity Value	0.439
Molecular Weight (kDa)	31.8
Isoelectric Point (pI)	10.07

Subcellular localization

Individual Mappings	Localization Confidence PMID Cytoplasmic Membrane Class 3 20472543
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession	Header	Accession Date	Compound	Source	Resolution	Method	Percent Identity
2GQC	HYDROLASE	04/20/06	Solution structure of the N-terminal domain of Rhomboid Intramembrane Protease from P. aeruginosa	Pseudomonas aeruginosa	NOT	SOLUTION NMR	100.0

Pathogen Association Analysis

Results

Common Found in both pathogen and nonpathogenic strains Hits to this gene were found in 163 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database	View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group	POG002506 (533 members)
Putative Inparalogs	None Found

Interactions

STRING database

Search for predicted protein-protein interactions using: Search term: PA3086 Search term: glpG Search term: rhomboid family protease GlpG

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen. Lewenza S, Gardy JL, Brinkman FS, Hancock RE Genome Res 2005 Feb;15(2):321-9 PubMed ID: 15687295

Solution structure and dynamics of the N-terminal cytosolic domain of rhomboid intramembrane protease from Pseudomonas aeruginosa: insights into a functional role in intramembrane proteolysis. Del Rio A, Dutta K, Chavez J, Ubarretxena-Belandia I, Ghose R J Mol Biol 2007 Jan 5;365(1):109-22 PubMed ID: 17059825

Insights into the effect of detergents on the full-length rhomboid protease from Pseudomonas aeruginosa and its cytosolic domain. Sherratt AR, Braganza MV, Nguyen E, Ducat T, Goto NK Biochim Biophys Acta 2009 Nov;1788(11):2444-53 PubMed ID: 19761755