Pseudomonas aeruginosa PAO1, PA3107 (metZ)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3107
Name
metZ
Replicon chromosome
Genomic location 3487181 - 3488392 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251797.1
GI 15598303
Affymetrix PA3107_metZ_at
Entrez 880476
GenBank AAG06495.1
INSDC AAG06495.1
NCBI Locus Tag PA3107
protein_id(GenBank) gb|AAG06495.1|AE004735_2|gnl|PseudoCAP|PA3107
TIGR NTL03PA03107
UniParc UPI000012F01D
UniProtKB Acc P55218
UniProtKB ID METZ_PSEAE
UniRef100 UniRef100_P55218
UniRef50 UniRef50_P55218
UniRef90 UniRef90_P55218

Product

Feature Type CDS
Coding Frame 1
Product Name
o-succinylhomoserine sulfhydrylase
Product Name Confidence: Class 1
Synonyms
Evidence for Translation
Detected by MALDI-TOF/TOF (PMID:19333994).
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -3.95
Kyte-Doolittle Hydrophobicity Value -0.038
Molecular Weight (kDa) 43.2
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 567 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002491 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3107
Search term: metZ

Human Homologs

Ensembl 110, assembly GRCh38.p14
cystathionine gamma-lyase [Source:HGNC Symbol;Acc:HGNC:2501]
E-value: 2.1e-61
Percent Identity: 38.0

References

A direct sulfhydrylation pathway is used for methionine biosynthesis in Pseudomonas aeruginosa.
Foglino M, Borne F, Bally M, Ball G, Patte JC
Microbiology (Reading) 1995 Feb;141 ( Pt 2):431-9
PubMed ID: 7704274
Pathways of assimilative sulfur metabolism in Pseudomonas putida.
Vermeij P, Kertesz MA
J Bacteriol 1999 Sep;181(18):5833-7
PubMed ID: 10482527
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994