Pseudomonas aeruginosa PAO1, PA3121 (leuC)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3121
Name
leuC
Replicon chromosome
Genomic location 3503292 - 3504716 (- strand)
Transposon Mutants 1 transposon mutants in PAO1
Transposon Mutants in orthologs 3 transposon mutants in orthologs

Cross-References

RefSeq NP_251811.1
GI 15598317
Affymetrix PA3121_leuC_at
Entrez 882827
GenBank AAG06509.1
INSDC AAG06509.1
NCBI Locus Tag PA3121
protein_id(GenBank) gb|AAG06509.1|AE004736_6|gnl|PseudoCAP|PA3121
TIGR NTL03PA03121
UniParc UPI000012E509
UniProtKB Acc Q9HZA3
UniProtKB ID LEUC_PSEAE
UniRef100 UniRef100_B7VBP9
UniRef50 UniRef50_P80858
UniRef90 UniRef90_Q884C2

Product

Feature Type CDS
Coding Frame 1
Product Name
3-isopropylmalate dehydratase large subunit
Product Name Confidence: Class 2
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) -5.36
Kyte-Doolittle Hydrophobicity Value -0.166
Molecular Weight (kDa) 51.0
Isoelectric Point (pI) 6.25

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 619 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002477 (534 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3121
Search term: leuC

Human Homologs

References

Hydrophobic interaction at the subunit interface contributes to the thermostability of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus.
Kirino H, Aoki M, Aoshima M, Hayashi Y, Ohba M, Yamagishi A, Wakagi T, Oshima T
Eur J Biochem 1994 Feb 15;220(1):275-81
PubMed ID: 8119295