Pseudomonas aeruginosa PAO1, PA3227 (ppiA)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3227
Name
ppiA
Synonym: cypH
Replicon chromosome
Genomic location 3614304 - 3614867 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251917.1
GI 15598423
Affymetrix PA3227_ppiA_at
DNASU PaCD00007420
Entrez 882464
GenBank AAG06615.1
INSDC CAA58868.1
NCBI Locus Tag PA3227
protein_id(GenBank) gb|AAG06615.1|AE004745_12|gnl|PseudoCAP|PA3227
TIGR NTL03PA03227
UniParc UPI000013206E
UniProtKB Acc Q59641
UniProtKB ID PPIA_PSEAE
UniRef100 UniRef100_Q59641
UniRef50 UniRef50_O53021
UniRef90 UniRef90_Q59641

Product

Feature Type CDS
Coding Frame 1
Product Name
peptidyl-prolyl cis-trans isomerase A
Product Name Confidence: Class 2
Synonyms Rotamase A, PPIase A
Cyclophilin A
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by two-dimensional gel electrophoresis and mass spectral analysis(PMID:21863142).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) 1.68
Kyte-Doolittle Hydrophobicity Value 0.119
Molecular Weight (kDa) 20.1
Isoelectric Point (pI) 8.46

Subcellular localization

Individual Mappings
Localization Confidence PMID
Periplasmic Class 3
Periplasmic Class 1
Additional evidence for subcellular localization
Detetected in a periplasmic protein preparation by spheroplasting by lysozyme and MgCl2.(PMID:19333994)

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 553 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002387 (537 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3227
Search term: ppiA

Human Homologs

Ensembl 110, assembly GRCh38.p14
peptidylprolyl isomerase domain and WD repeat containing 1 [Source:HGNC Symbol;Acc:HGNC:28954]
E-value: 1.3e-16
Percent Identity: 36.8
Ensembl 110, assembly GRCh38.p14
peptidylprolyl isomerase domain and WD repeat containing 1 [Source:HGNC Symbol;Acc:HGNC:28954]
E-value: 1.3e-16
Percent Identity: 36.8
Ensembl 110, assembly GRCh38.p14
peptidylprolyl isomerase domain and WD repeat containing 1 [Source:HGNC Symbol;Acc:HGNC:28954]
E-value: 1.3e-16
Percent Identity: 36.8

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Two distinct forms of peptidylprolyl-cis-trans-isomerase are expressed separately in periplasmic and cytoplasmic compartments of Escherichia coli cells.
Hayano T, Takahashi N, Kato S, Maki N, Suzuki M
Biochemistry 1991 Mar 26;30(12):3041-8
PubMed ID: 2007139
Three-dimensional solution structure of Escherichia coli periplasmic cyclophilin.
Clubb RT, Ferguson SB, Walsh CT, Wagner G
Biochemistry 1994 Mar 15;33(10):2761-72
PubMed ID: 8130188
Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome.
Liao X, Charlebois I, Ouellet C, Morency MJ, Dewar K, Lightfoot J, Foster J, Siehnel R, Schweizer H, Lam JS, Hancock REW, Levesque RC
Microbiology (Reading) 1996 Jan;142 ( Pt 1):79-86
PubMed ID: 8581173
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994
Comparisons of Two Proteomic Analyses of Non-Mucoid and Mucoid Pseudomonas aeruginosa Clinical Isolates from a Cystic Fibrosis Patient.
Rao J, Damron FH, Basler M, Digiandomenico A, Sherman NE, Fox JW, Mekalanos JJ, Goldberg JB
Front Microbiol 2011;2:162
PubMed ID: 21863142