Pseudomonas aeruginosa PAO1, PA3250

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3250
Name
Replicon chromosome
Genomic location 3636621 - 3637679 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_251940.1
GI 15598446
Affymetrix PA3250_at
Entrez 882413
GenBank AAG06638.1
INSDC AAG06638.1
NCBI Locus Tag PA3250
protein_id(GenBank) gb|AAG06638.1|AE004747_11|gnl|PseudoCAP|PA3250
TIGR NTL03PA03250
UniParc UPI00000C5973
UniProtKB Acc Q9HYZ0
UniProtKB ID Q9HYZ0_PSEAE
UniRef100 UniRef100_Q9HYZ0
UniRef50 UniRef50_Q3KFM7
UniRef90 UniRef90_Q9HYZ0

Product

Feature Type CDS
Coding Frame 1
Product Name
hypothetical protein
Product Name Confidence: Class 4
Synonyms
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected by MALDI-TOF/TOF (PMID:19333994).
Charge (pH 7) -2.03
Kyte-Doolittle Hydrophobicity Value -0.312
Molecular Weight (kDa) 38.3
Isoelectric Point (pI) 6.17

Subcellular localization

Individual Mappings
Localization Confidence PMID
Unknown Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 158 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002359 (513 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3250

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Analysis of the periplasmic proteome of Pseudomonas aeruginosa, a metabolically versatile opportunistic pathogen.
Imperi F, Ciccosanti F, Perdomo AB, Tiburzi F, Mancone C, Alonzi T, Ascenzi P, Piacentini M, Visca P, Fimia GM
Proteomics 2009 Apr;9(7):1901-15
PubMed ID: 19333994