Pseudomonas aeruginosa PAO1, PA3262

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3262
Name
Replicon chromosome
Genomic location 3649705 - 3650466 (- strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251952.1
GI 15598458
Affymetrix PA3262_at
Entrez 882425
GenBank AAG06650.1
INSDC AAG06650.1
NCBI Locus Tag PA3262
protein_id(GenBank) gb|AAG06650.1|AE004748_8|gnl|PseudoCAP|PA3262
TIGR NTL03PA03262
UniParc UPI00000C597F
UniProtKB Acc Q9HYX8
UniProtKB ID Q9HYX8_PSEAE
UniRef100 UniRef100_W0YVN7
UniRef50 UniRef50_A4VN69
UniRef90 UniRef90_A6V2F7

Product

Feature Type CDS
Coding Frame 1
Product Name
probable peptidyl-prolyl cis-trans isomerase, FkbP-type
Product Name Confidence: Class 3
Synonyms
Evidence for Translation
Verified using two-dimensional gel electrophoresis, MALDI-TOF MS and peptide mass mapping(pI:5.1, molecular weight:26.8, PMID:21674802)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (26.85 kDa, PMID:21751344)
Charge (pH 7) -7.30
Kyte-Doolittle Hydrophobicity Value -0.348
Molecular Weight (kDa) 26.8
Isoelectric Point (pI) 4.84

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Class 2
Cytoplasmic Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

PDB 3D Structures

Accession Header Accession Date Compound Source Resolution Method Percent Identity
7DEK ISOMERASE 11/04/20 Pseudomonas aeruginosa FK506-binding protein PaFkbA Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 2.9 X-RAY DIFFRACTION 100.0

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 398 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002348 (536 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3262

Human Homologs

Ensembl 110, assembly GRCh38.p14
FKBP prolyl isomerase 1A [Source:HGNC Symbol;Acc:HGNC:3711]
E-value: 3.6e-14
Percent Identity: 42.1
Ensembl 110, assembly GRCh38.p14
FKBP prolyl isomerase 1A [Source:HGNC Symbol;Acc:HGNC:3711]
E-value: 3.6e-14
Percent Identity: 42.1
Ensembl 110, assembly GRCh38.p14
FKBP prolyl isomerase 1A [Source:HGNC Symbol;Acc:HGNC:3711]
E-value: 3.6e-14
Percent Identity: 42.1
Ensembl 110, assembly GRCh38.p14
FKBP prolyl isomerase 1A [Source:HGNC Symbol;Acc:HGNC:3711]
E-value: 3.6e-14
Percent Identity: 42.1
Ensembl 110, assembly GRCh38.p14
FKBP prolyl isomerase 1A [Source:HGNC Symbol;Acc:HGNC:3711]
E-value: 3.6e-14
Percent Identity: 42.1

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Isolation and amino acid sequence of a new 22-kDa FKBP-like peptidyl-prolyl cis/trans-isomerase of Escherichia coli. Similarity to Mip-like proteins of pathogenic bacteria.
Rahfeld JU, Rücknagel KP, Stoller G, Horne SM, Schierhorn A, Young KD, Fischer G
J Biol Chem 1996 Sep 6;271(36):22130-8
PubMed ID: 8703024
Proteomics of the oxidative stress response induced by hydrogen peroxide and paraquat reveals a novel AhpC-like protein in Pseudomonas aeruginosa.
Hare NJ, Scott NE, Shin EH, Connolly AM, Larsen MR, Palmisano G, Cordwell SJ
Proteomics 2011 Aug;11(15):3056-69
PubMed ID: 21674802
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344