Pseudomonas aeruginosa PAO1, PA3271

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3271
Name
Replicon chromosome
Genomic location 3660022 - 3663501 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 2 transposon mutants in orthologs

Cross-References

RefSeq NP_251961.1
GI 15598467
Affymetrix PA3271_at
Entrez 882434
GenBank AAG06659.1
INSDC AAG06659.1
NCBI Locus Tag PA3271
protein_id(GenBank) gb|AAG06659.1|AE004749_8|gnl|PseudoCAP|PA3271
TIGR NTL03PA03271
UniParc UPI00000C5986
UniProtKB Acc Q9HYX0
UniProtKB ID Q9HYX0_PSEAE
UniRef100 UniRef100_Q9HYX0
UniRef50 UniRef50_Q4K8D2
UniRef90 UniRef90_Q9HYX0

Product

Feature Type CDS
Coding Frame 1
Product Name
probable two-component sensor
Product Name Confidence: Class 3
Synonyms probable sensory transduction histidine kinase
Evidence for Translation
Detected using LC-MS/MS (127.45 kDa, PMID:21751344)
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 8.34
Kyte-Doolittle Hydrophobicity Value 0.220
Molecular Weight (kDa) 127.5
Isoelectric Point (pI) 8.22

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Membrane Class 3
Outer Membrane Vesicle Class 1
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 617 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002342 (960 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3271

Human Homologs

References

Genome-wide identification of Pseudomonas aeruginosa exported proteins using a consensus computational strategy combined with a laboratory-based PhoA fusion screen.
Lewenza S, Gardy JL, Brinkman FS, Hancock RE
Genome Res 2005 Feb;15(2):321-9
PubMed ID: 15687295
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344