Pseudomonas aeruginosa PAO1, PA3299 (fadD1)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3299
Name
fadD1
Replicon chromosome
Genomic location 3695511 - 3697199 (- strand)
Transposon Mutants 4 transposon mutants in PAO1
Transposon Mutants in orthologs 4 transposon mutants in orthologs

Cross-References

RefSeq NP_251989.1
GI 15598495
Affymetrix PA3299_fadD1_at
Entrez 882462
GenBank AAG06687.1
INSDC AAG06687.1
NCBI Locus Tag PA3299
protein_id(GenBank) gb|AAG06687.1|AE004752_3|gnl|PseudoCAP|PA3299
TIGR NTL03PA03299
UniParc UPI00000C599D
UniProtKB Acc Q9HYU4
UniProtKB ID Q9HYU4_PSEAE
UniRef100 UniRef100_W0YVT1
UniRef50 UniRef50_Q8ZES9
UniRef90 UniRef90_A0A0A8RC61

Product

Feature Type CDS
Coding Frame 1
Product Name
long-chain-fatty-acid--CoA ligase
Product Name Confidence: Class 2
Synonyms acyl coenzyme A synthetase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Detected using LC-MS/MS (61.65 kDa, PMID:21751344)
Charge (pH 7) 2.08
Kyte-Doolittle Hydrophobicity Value -0.027
Molecular Weight (kDa) 61.7
Isoelectric Point (pI) 7.45

Subcellular localization

Individual Mappings
Localization Confidence PMID
Outer Membrane Vesicle Class 1
Cytoplasmic Class 2
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 601 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002317 (1059 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3299
Search term: fadD1

Human Homologs

Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase medium chain family member 2B [Source:HGNC Symbol;Acc:HGNC:30931]
E-value: 1.3e-37
Percent Identity: 28.7
Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase medium chain family member 2B [Source:HGNC Symbol;Acc:HGNC:30931]
E-value: 1.3e-37
Percent Identity: 28.7
Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase medium chain family member 2B [Source:HGNC Symbol;Acc:HGNC:30931]
E-value: 1.3e-37
Percent Identity: 28.7
Ensembl 110, assembly GRCh38.p14
acyl-CoA synthetase medium chain family member 2B [Source:HGNC Symbol;Acc:HGNC:30931]
E-value: 1.3e-37
Percent Identity: 28.7

References

Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase.
Black PN, DiRusso CC, Metzger AK, Heimert TL
J Biol Chem 1992 Dec 15;267(35):25513-20
PubMed ID: 1460045
Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa.
Choi DS, Kim DK, Choi SJ, Lee J, Choi JP, Rho S, Park SH, Kim YK, Hwang D, Gho YS
Proteomics 2011 Aug;11(16):3424-9
PubMed ID: 21751344
Multiple FadD acyl-CoA synthetases contribute to differential fatty acid degradation and virulence in Pseudomonas aeruginosa.
Kang Y, Zarzycki-Siek J, Walton CB, Norris MH, Hoang TT
PLoS One 2010 Oct 21;5(10):e13557
PubMed ID: 21042406