Pseudomonas aeruginosa PAO1, PA3495 (nth)

Cytoplasmic
Cytoplasmic Membrane
Periplasmic
Outer Membrane
Extracellular
Unknown
View in JBrowse PseudoCyc / Metabolic Pathways

Gene Feature Overview

Strain Pseudomonas aeruginosa PAO1 (Stover et al., 2000)
GCF_000006765.1|latest
Locus Tag
PA3495
Name
nth
Replicon chromosome
Genomic location 3913129 - 3913767 (+ strand)
Transposon Mutants 2 transposon mutants in PAO1
Transposon Mutants in orthologs 1 transposon mutants in orthologs

Cross-References

RefSeq NP_252185.1
GI 15598691
Affymetrix PA3495_nth_at
DNASU PaCD00007536
Entrez 879069
GenBank AAG06883.1
INSDC AAG06883.1
NCBI Locus Tag PA3495
protein_id(GenBank) gb|AAG06883.1|AE004770_8|gnl|PseudoCAP|PA3495
TIGR NTL03PA03495
UniParc UPI00000C5A37
UniProtKB Acc Q9HYB4
UniProtKB ID Q9HYB4_PSEAE
UniRef100 UniRef100_W0YVV1
UniRef50 UniRef50_P44319
UniRef90 UniRef90_A6V1T3

Product

Feature Type CDS
Coding Frame 1
Product Name
endonuclease III
Product Name Confidence: Class 2
Synonyms deoxyribonuclease
DNA lyase
Evidence for Translation
Identified using nanoflow high-pressure liquid chromatography (HPLC) in conjunction with microelectrospray ionization on LTQ XL mass spectrometer (PMID:24291602).
Charge (pH 7) 5.77
Kyte-Doolittle Hydrophobicity Value -0.282
Molecular Weight (kDa) 23.7
Isoelectric Point (pI) 8.71

Subcellular localization

Individual Mappings
Localization Confidence PMID
Cytoplasmic Class 3
Additional evidence for subcellular localization

AlphaFold 2 Protein Structure Predictions

Protein structure predictions using a neural network model developed by DeepMind. If a UniProtKB accession is associated with this protein, a search link will be provided below.

Look for predicted 3D structure in AlphaFold DB: Search

Pathogen Association Analysis

Results
Common
Found in both pathogen and nonpathogenic strains
Hits to this gene were found in 651 genera

Orthologs/Comparative Genomics

Pseudomonas Ortholog Database View orthologs at Pseudomonas Ortholog Database
Pseudomonas Ortholog Group POG002149 (535 members)
Putative Inparalogs None Found

Interactions

STRING database Search for predicted protein-protein interactions using:
Search term: PA3495
Search term: nth
Search term: endonuclease III

Human Homologs

References

Purification and characterization of Escherichia coli endonuclease III from the cloned nth gene.
Asahara H, Wistort PM, Bank JF, Bakerian RH, Cunningham RP
Biochemistry 1989 May 16;28(10):4444-9
PubMed ID: 2669955
Atomic structure of the DNA repair [4Fe-4S] enzyme endonuclease III.
Kuo CF, McRee DE, Fisher CL, O'Handley SF, Cunningham RP, Tainer JA
Science 1992 Oct 16;258(5081):434-40
PubMed ID: 1411536
Structure and function of Escherichia coli endonuclease III.
Cunningham RP, Ahern H, Xing D, Thayer MM, Tainer JA
Ann N Y Acad Sci 1994 Jul 29;726:215-22
PubMed ID: 8092678